Status:
ReviewerMember since: 2016
Organization: Vertex Pharmaceuticals
Excellent results and good reproducibility
Application Area: High throughput screening
"Very high quality reagents, easy to use with good instructions, Individual components can be purchased separately."
The FLIPR Potassium Assay Kit exploits the permeability of thallium ions (Tl+) through both voltage- and ligand-gated potassium (K+) channels.
In this assay, a novel, highly-sensitive Tl+ indicator dye is utilized which produces a bright fluorescent signal upon the binding to Tl+ conducted through potassium channels. The intensity of the Tl+ signal is proportional to the number of potassium channels in the open state; therefore it provides a functional indication of the potassium channel activities. In addition, one of our proprietary masking dyes is employed to further reduce background fluorescence for improved signal/noise ratio.