Mix-and-read simplicity to rapidly screen ion channel targets.
The FLIPR® Membrane Potential Assay Kits deliver homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins.
Each homogeneous assay kit utilizes a proprietary indicator dye and quencher combination to maximize cell line/channel/compound applicability, while eliminating causes of data variability. This unique formula responds 10 times faster and has greater temperature stability than traditional dyes, providing high quality screening data that shows good correlation with manual patch clamp assays.
Because ion channel activity is highly sensitive and potentially impacted by subtle chemical changes, two FLIPR® Membrane Potential Assay Kits (Red and Blue) are available to select the optimal conditions for your delicate ion channel targets. Both formulations utilize Molecular Devices' proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels.
Unlike traditional dyes such as DiBAC, FLIPR Membrane Potential Assay Kits detect bidirectional gradient changes so you can monitor both variable and control conditions within a single experiment. We recommend evaluating both assay kits to discern which formulation is right for your target.