Covaris FFPE Nucleic Acid Extraction Kit provides an optimized reagent and protocol for extracting DNA from FFPE tissue samples. Adaptive Focused Acoustic™ (AFA) Technology is used to emulsify and separate the paraffin, while simultaneously re-hydrating the tissue sample in an aqueous solution. Covaris Focused-ultrasonicators enable the purely mechanical processing of the samples, eliminating the need for organic solvents. Large Molecular Weight DNA is efficiently extracted from FFPE samples, with greater yield compared to organic extraction with other commercially available kits.
The FFPE Nucleic Acid Extraction Kit utilizes AFA Technology, the standard for DNA fragmentation for Next Generation Sequencing (NGS). An optional DNA fragmentation step can be included in the workflow to precisely size the DNA for applications requiring short DNA fragments, such as NGS – all in the same tube. This optional integrated single-tube DNA Shearing streamlines the extraction process to analytical method workflow, while also enhancing total DNA yield, 2X the yield compared to extraction without fragmentation.