Fast Trap Adenovirus Purification and Concentration Kit by Merck

The Fast-Trap Adenovirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification of ≥ 3 mL of adenovirus serotype 5, up to a capacity of approximately 1x1013 total adenovirus particles.

The purification kit protocol calls for releasing the virus from the infected cells using multiple freeze-thaw cycles, then collecting the crude adenovirus. Most of the large cellular debris is removed by centrifugation, leaving the viable virus particles in the supernatant. The supernatant is further clarified by passing it through a Steriflip^®-HV 0.45 µm filter. After adding a binding buffer, the virus solution is passed over a treated filter that adsorbs the virus particles, allowing the smaller cellular debris and media to pass through the filter. Following a wash to remove any weakly bound proteins, the virus is eluted off the filter with an elution buffer. Finally, the virus can be concentrated and exchanged into the desired buffer using an Amicon^® Ultra centrifugal filter unit.

For laboratory use only; not for diagnostic or in vivo use.