FAST Quant®, Microspot ELISA for high-troughput multiplex cytokine quantification 

FAST Quant represents a quantum leap forward in protein microarray technology. With FAST Quant a researcher can accurately determine the concentration of several cytokines in dozens of biological samples simultaneously, using familiar ELISA immunochemistry. Each FAST Quant kit contains 64 arrays of monoclonal antibodies with affinities for common human or mouse cytokines. The antibodies are arrayed in a quantitative fashion in triplicate on each array.

Using four 16 pad FAST Slides placed in the FAST Frame, FAST Quant offers an 8 x 12 cm footprint - the same as the traditional microplate format. A standard curve can be generated by creating a dilutional series from recombinant antigen mass standard.

Because of the solid-phase nature of a microspot assay, it is not necessary to take duplicate measurements or each sample. The MicroSpot ELISA reaction is entirely concentration dependent, unlike an ELISA where the reaction is both concentration- and volume-dependent.

FAST Quant antibody arrays offer a variety of human and mouse menus. All arrays come in a four-slide kit with recombinant antigen standard, detection antibodies and processing buffers.

FAST Quant Mouse Th1/Th2 panel: IL-1ß, IL-2, IL-4, IL-5, TNFalpha, IL-6, IL-10, IL-13, IFNgamma