FAM FLIVO® in vivo Apoptosis Kit by Immunochemistry Technologies LLC

The non-cytotoxic FLIVO (FLuorescence in vIVO) probes are injectable fluorescent inhibitors of caspase activity. Upon intravenous injection, the FLIVO® probes preferentially form covalent bonds with active caspases, marking cells undergoing apoptosis with fluorescence.

FAM FLIVO® has a green fluorescent label, carboxyfluorescein, with an optimal excitation range from 490 - 495 nm and optimal emission range from 515 - 525 nm. FLIVO is also available with a visible red fluorescent label and two near-infrared (NIR) fluorescent labels. Products are supplied in small kits (enough for 6 mice or rats) and large kits (enough for 24 mice or rats).

FLIVO in vivo Apoptosis Kits may be used in animal models to monitor the efficacy of treatment or the effects of disease. Among its demonstrated uses, it has been used successfully in murine models of cancer, rat brain studies, and avian brain studies. FLIVO® is non-toxic, cell-permeant, and crosses the blood-brain barrier. Reagent titration experiments should be performed to determine the amount of reagent that will work best for the size of the animal and the target tissue type.