The EZ-Zyme™ Chromatin Prep kit allows ChIP analysis at nucleosome resolution by performing complete or partial digestions with a proprietary enzymatic cocktail to obtain chromatin fragments of on average one to a few nucleosomes in length. Compared to sonication, this procedure not only allows mapping the protein-DNA association at a higher resolution, but also renders the subsequent immunoprecipitation more efficient due to its mild digestion condition. In addition, enzymatic shearing is compatible with non-crosslinked ChIP (native ChIP), which allows analysis of histone modifications not only on cultured cells, but also on freshly dissected and frozen tissues. The EZ-Zyme™ kit contains reagents optimized for generating enzymatically processed chromatin from mammalian cells for use in c...Read more
Receive your quote directly from EMD Millipore, a division of Merck KGaA, Darmstadt, Germany.