Expi293™ Expression System Kit by Thermo Fisher Scientific Invitrogen

The Expi293™ Expression System is a major advance in transient expression technology for rapid and ultra high-yield protein production from mammalian cells. It is based on high density culture of Expi293F™ cells in Expi293™ Expression Medium.

Transient expression is powered by the cationic lipid-based ExpiFectamine™ 293 transfection reagent in combination with specialized transfection enhancers. All components work in concert to generate 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle™ 293 Expression System. Expression yields of >1 gram per liter of transfected culture have been demonstrated for some antibody and non-antibody proteins.


Features:

• Rapid production of milligram to gram quantities of recombinant protein in mammalian cells
• High density cultures of Expi293F™ cells that result in more expressing cells per milliliter of culture and higher protein yields
• Native folding and mammalian post-translational modifications, such as glycosylation, of expressed proteins
• Easy, robust culture and transfection protocols that readily fit into current transient expression workflows
• Scalable expression that can be applied to cultures from<1 mL to >10 L, thus generating the amount of protein needed