Epigenetic Chromatin Modification Enzymes PCR Array by QIAGEN

The Human Epigenetic Chromatin Modification Enzymes RT² Profiler™ PCR Array profiles the expression of 84 key genes encoding enzymes known or predicted to modify genomic DNA and histones to regulate chromatin accessibility and therefore gene expression. The de novo and maintenance DNA methyltransferases, and the enzymes responsible for demethylation of CpG dinucleotides are represented by the array. Enzymes catalyzing histone acetylation, methylation, phosphorylation, and ubiquitination are also included on the array as well as the enzymes the deacetylases and demethylases. The array also analyzes genes encoding the SET domain proteins, which all contain a homologous domain that demonstrates histone methyltransferase activity in some family members. During the development of stem cells to terminally differentiated cells, altered expression occurs for many of these genes to control chromatin dynamics. These genes also exhibit different expression profiles in tumor cells relative to normal cells, suggesting a role for chromatin modification and remodeling in oncogenesis. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of genes involved in epigenetic chromatin modifications with this array.