Enzymatic Chromatrap® Standard Pro-A spin Column Shearing Kit by Chromatrap
Manufacturer Chromatrap
24 Chromatrap® Protein-A based spin-columns, buffers and reagents for enzymatic shearing of chromatin
The Chromatin immunoprecipitation (ChIP) assay is a technique used to investigate the interaction between proteins and DNA in the cell. For example, it may be used to determine whether proteins such as transcription factors and modified histones bind to a particular region on the endogenous chromatin of living cells or tissues. In a ChIP assay, fragments of the DNA-protein complex (i.e. chromat... Read more
The supplier does not provide quotations for this product through SelectScience. Browse these related products instead
Related Products
Enzymatic Chromatrap® Standard Pro-A spin Column Shearing Kit
24 Chromatrap® Protein-A based spin-columns, buffers and reagents for enzymatic shearing of chromatin
The Chromatin immunoprecipitation (ChIP) assay is a technique used to investigate the interaction between proteins and DNA in the cell. For example, it may be used to determine whether proteins such as transcription factors and modified histones bind to a particular region on the endogenous chromatin of living cells or tissues.
In a ChIP assay, fragments of the DNA-protein complex (i.e. chromatin) are prepared in such a way so as to retain the specific DNA-protein interactions. These fragments are captured on an in inert porous solid phase disc using an immobilized antibody specific for the protein present in the complex. The isolated chromatin fraction can then be cleaned up, released into fresh buffers and treated to separate the DNA and protein components. The identity of DNA fragments isolated in connection with a particular protein can then be determined by Polymerase Chain Reaction (PCR), Real Time PCR, hybridization on microarrays, direct sequencing or other technologies used for identification of DNA fragments.
The kit contains sufficient Protein-A based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and upto 10 chromatin preparations.
Enzymatic Chromatrap® Standard Pro-A spin Column Shearing Kit Content:
The Chromatin immunoprecipitation (ChIP) assay is a technique used to investigate the interaction between proteins and DNA in the cell. For example, it may be used to determine whether proteins such as transcription factors and modified histones bind to a particular region on the endogenous chromatin of living cells or tissues.
In a ChIP assay, fragments of the DNA-protein complex (i.e. chromatin) are prepared in such a way so as to retain the specific DNA-protein interactions. These fragments are captured on an in inert porous solid phase disc using an immobilized antibody specific for the protein present in the complex. The isolated chromatin fraction can then be cleaned up, released into fresh buffers and treated to separate the DNA and protein components. The identity of DNA fragments isolated in connection with a particular protein can then be determined by Polymerase Chain Reaction (PCR), Real Time PCR, hybridization on microarrays, direct sequencing or other technologies used for identification of DNA fragments.
The kit contains sufficient Protein-A based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and upto 10 chromatin preparations.
Enzymatic Chromatrap® Standard Pro-A spin Column Shearing Kit Content:
- Chromatrap® spin columns 24
- Column conditioning buffer 50mls
- Wash Buffer 1 50mls
- Wash Buffer 2 50mls
- Wash Buffer 3 50mls
- 1.3M Glycine 20mls
- Lysis Buffer 10mls
- Digestion Buffer 10mls
- Enzymatic Stop Solution 200µl
- 0.1M Elution Solution 3mls
- 0.1M NaOH 3mls
- 5M NaCl 500µl
- 1M NaHCO3 500µl
- Hypotonic Buffer 10mls
- Shearing Cocktail 100µl
- Protease Inhibitor Cocktail (PIC) 100µl
- Proteinase K stop solution 100µl
- Proteinase K 50µl
- 1.5ml Collection tube 24
Product Overview
Enzymatic Chromatrap® Standard Pro-A spin Column Shearing Kit
Manufacturer Chromatrap
Be the first to review this product