Enzymatic Chromatrap® Premium Pro-G Spin Column Shearing Kit by Chromatrap

The Chromatin immunoprecipitation (ChIP) assay is a technique used to investigate the interaction between proteins and DNA in the cell. For example, it may be used to determine whether proteins such as transcription factors and modified histones bind to a particular region on the endogenous chromatin of living cells or tissues.

The kit contains sufficient Protein-G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.

This Premium kit also includes the following reference standards:
Positive Antibody Control: RNA Polymerase II (H - 244) Rabbit polyclonal
Negative Antibody Control: IgG from Rabbit serum 0.2µg/µl
Forward primer: GAPDH (human promoter region)
Reverse primer: GAPDH (human promoter region)


Enzymatic Chromatrap® Premium Pro-G Spin Column Shearing Kit Contents:

• Chromatrap® spin columns 24
• Column conditioning buffer 50mls
• Wash Buffer 1 50mls
• Wash Buffer 2 50mls
• Wash Buffer 3 50mls
• 1.3M Glycine 20mls
• Lysis Buffer 10mls
• Digestion Buffer 10mls
• Enzymatic Stop Solution 200µl
• 0.1M Elution Solution 3mls
• 0.1M NaOH 3mls
• 5M NaCl 500µl
• 1M NaHCO3 500µl
• Hypotonic Buffer 10mls
• Shearing Cocktail 100µl
• Protease Inhibitor Cocktail (PIC) 100µl
• Proteinase K stop solution 100µl
• Proteinase K 50µl
• 1.5ml Collection tube 24