LI-COR ELISA HRP Substrate (680) and ELISA AP Substrate (680) provide excellent signal-to-noise, consistency, and linearity, making them ideal for use in most ELISA applications.
These substrates offer equal or better sensitivity as compared to commercially-available chromogenic and chemiluminescent substrates and are ideal for endpoint assays. The alkaline phosphatase (AP) substrate results in a colorless to light blue product in the presence of alkaline phosphatase. The reaction is stopped with a colored phosphatase stop solution, so the wells appear purple after the addition of stop solution.