Elastase by Worthington Biochemical Corp.

Elastase is effective in the isolation of Type II lung cells. Because elastin is found in the highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues which contain extensive intercellular fiber networks. The viscous extracellular ground substance in which connective tissue cells and fibers are embedded is a complex mixture of various glycoproteins. Given the complexity of this matrix, proteolytic digestion proceeds best when elastase is used in concert with other enzymes such as collagenase, trypsin and chymotrypsin.

Worthington also produces a lyophilized form of our widely used crystalline elastase preparation (Code:
ESL). This product can be used in place of the aqueous suspension preparation for tissue dissociation and cell isolation applications. It offers the convenience, stability, and versatility of a lyophilized powder along with the enzymatic activity of the liquid preparation.