The eFluor® NC Flow Cytometry Staining Buffer is designed for use in immunofluorescent staining protocols which include eFluor® Nanocrystal conjugated antibodies. Use of this buffer results in optimal eFluor® Nanocrystal antibody staining, but is also fully compatible with staining of conventional fluorochrome conjugated antibodies. eFluor® NC Flow Cytometry Staining Buffer is a buffered saline solution containing sodium azide (0.09%) as a preservative. eFluor® NC Flow Cytometry Staining Buffer is supplied as a 5X concentrated stock solution. The 5X stock solution should be diluted with distilled water to a 1X solution. It does not contain a source of protein and therefore, should be supplemented with 3% fetal bovine serum (final concentration) or equivalent protein prior to use. Thi...Read more
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