Easy-A high-fidelity PCR cloning enzyme combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A PCR cloning enzyme can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.
Combines the cloning efficiency of Taq DNA polymerase with the accuracy of Pfu DNA polymerase
Proofreading DNA polymerase formulation efficiently adds 3€™-A overhangs to PCR amplicons as efficiently as Taq
Easy-A-amplified PCR products are ready to clone into the TOPO TA Cloning vector and other T/U vectors
Fidelity equivalent to Pfu DNA polymerase and 6x more accurate than Taq DNA polymerase