Status:
ReviewerMember since: 2019
Organization: University of Maryland
Good and reliable results.
Application Area: Viral neutralization assay
"It's a well established system and there are many references to support the assay. Therefore it's pretty reliable and the results were pretty reproducible. The price was fair but still high cost considering our high sample amount."
Status:
ReviewerMember since: 2019
Organization: Earli
Excellent product, but a bit pricey.
Application Area:Characterize promoter activity
"Kit works great, we scale down the volumes a bit since the reagents are so expensive. We also have the GloMax plate reader which is excellent to use."
Status:
ReviewerMember since: 2019
Organization: UTHSCSA
Your products are very practical, good quality, and high efficiency.
Application Area:Caspase-glo3/7 assay
"A high sensitivity caspase assay that requires fewer cells and less enzyme."
Status:
ReviewerMember since: 2019
Organization: University of Colorado
Easy to use and effcient.
Application Area:Molecular biology/neuroscience
"This product is awesome. It is easy to use and reliable. However, I do think it is highly priced."
Status:
ReviewerMember since: 2011
Organization: UT MD ANDERSON CANCER CTR.
"This kit is expensive and not very easy to use, but is necessary for some of our research as there are not a lot of kits like this in the market."
The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors. In the DLR™ Assay System, both reporters yield linear assays with attomole (<10−18) sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ Assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.
The pGL4 and phRL series of synthetic Renilla Luciferase Reporter Vectors are designed for use with the DLR™ Assay Systems. A Renilla luciferase vector with constitutive expression may be used in combination with any experimental firefly luciferase vector to co-transfect mammalian cells.