Dual-Luciferase® Reporter Assay System

 4.4/5.0 (6 reviews)

Promega Corp.

The Dual-Luciferase ® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Ren...read more

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Reviews

YONG QIN


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Member since 2011

Organization:
UT MD ANDERSON CANCER CTR.
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RATING: 3.0

  • 3 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 2 out of 5VALUE FOR MONEY




Review date: 12 Jul 2011

Dual-Luciferase® Reporter Assay System

"This kit is expensive and not very easy to use, but is necessary for some of our research as there are not a lot of kits like this in the market."

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Fei Xing


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Member since 2011

Organization:
Southern Illinois University
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 11 Jul 2011

Dual-Luciferase® Reporter Assay System

"Easy to use."

GUILLERMO PELUFFO


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Member since 2011

Organization:
INST. OF ONCOLOGY ANGEL H. ROFFO
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RATING: 3.6

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 06 Jul 2011

Dual-Luciferase® Reporter Assay System

"Really easy to use product, reproducible results."

JACQUELINE JOHNSON


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Member since 2011

Organization:
H. LEE MOFFITT CANCER CTR.
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 05 Jul 2011

Dual-Luciferase® Reporter Assay System

"Very easy to use and has a long shelf life."

Andy Wyche


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Member since 2007

Organization:
Neuromed
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 09 Jul 2007

Dual-Luciferase® Reporter Assay System

Taranjit Singh Gujral


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Member since 2007

Organization:
Queen's University
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 11 Jun 2007

Dual-Luciferase® Reporter Assay System

Description

 

The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors. In the DLR™ Assay System, both reporters yield linear assays with attomole (<10−18) sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ Assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.

The pGL4 and phRL series of synthetic Renilla Luciferase Reporter Vectors are designed for use with the DLR™ Assay Systems. A Renilla luciferase vector with constitutive expression may be used in combination with any experimental firefly luciferase vector to co-transfect mammalian cells.

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