Dual-Luciferase® Reporter Assay System

Manufacturer Promega Corp.
4.4
/
5.0
  |  6 reviews


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Average Rating: 4.4
6 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Rating: 3.0

"This kit is expensive and not very easy to use, but is necessary for some of our research as there are not a lot of kits like this in the market."

Review date: 12 Jul 2011 | Dual-Luciferase® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Southern Illinois University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7
"Easy to use."

Review date: 11 Jul 2011 | Dual-Luciferase® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: INST. OF ONCOLOGY ANGEL H. ROFFO



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7
"Really easy to use product, reproducible results."

Review date: 06 Jul 2011 | Dual-Luciferase® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: H. LEE MOFFITT CANCER CTR.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"Very easy to use and has a long shelf life."

Review date: 05 Jul 2011 | Dual-Luciferase® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Neuromed



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

Review date: 09 Jul 2007 | Dual-Luciferase® Reporter Assay System


  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Queen's University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

Review date: 11 Jun 2007 | Dual-Luciferase® Reporter Assay System
The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors. In the DLR™ Assay System, both reporters yield linear assays with attomole (<10−18) sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ Assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.

The pGL4 and phRL series of synthetic Renilla Luciferase Reporter Vectors are designed for use with the DLR™ Assay Systems. A Renilla luciferase vector with constitutive expression may be used in combination with any experimental firefly luciferase vector to co-transfect mammalian cells.

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Dual-Luciferase® Reporter Assay System

Manufacturer Promega Corp.

4.4 / 5.0 | 6 reviews