Status:
ReviewerMember since: 2023
Organization: Foundation for Research and Technology - Hellas
Good for necrotic nuclei, pity that Draq5 and Draq9 are at the same wavelengths.
Application Area: Visualize necrosis on glioblastoma primary spheroids with LSFM imaging
"Glioblastoma spheroids of ~1mm in diameter are incubated with Draq7 for 16-20hrs before imaging. Draq7 is visualized in far red and stains dead nuclei, without discriminating cell death pattern. Easily combined with blue and green probes. Photobleaches after 6hrs."
Status:
ReviewerMember since: 2023
Organization: University of Galway
Great results
Application Area:cell viability assessment
"The dye is perfect for detecting dead cells by flow cytometry. Stable, easy to use, quite sensitive."
Status:
ReviewerMember since: 2023
Organization: UNC Chapel Hill
Great, reproducible results
Application Area:Assess nuclei in 2D and 3D immunofluorescence
"We use this product to look at nuclei in both 2D and 3D fixed immunofluorescence imaging. Very useful if you do not have access to a 405 laser and cannot use DAPI, as this uses the 647 channel."
Status:
ReviewerMember since: 2022
Organization: University of California, Riverside
It works well, minimal crosstalk with other fluorescent channels
Application Area:Nuclear counter stain in deep red
"It works well, minimal crosstalk with other fluorescent channels. If you have weak signal use a higher concentration than recommended."
Status:
ReviewerMember since: 2021
Organization: University of Rhode Island
Great results!
Application Area:Detect double-strand DNA in tissue samples
"In our study, cell-impermeable DNA dye DRAQ7 was injected to explore potential host DNA release. This reagent helped us to prove our experiment could induce DNA release easily. We have published our paper!"
Status:
ReviewerMember since: 2021
Organization: KIYATEC, Inc.
Our staff prefer DRAQ7 for detecting dead cells using flow cytometry.
Application Area:Flow cytometry
"We have switched to using DRAQ7 for all of our flow cytometry experiments for the detection of dead cells."
Status:
ReviewerMember since: 2020
Organization: UCSF
Useful fixable DNA stain for assessments of cell membrane integrity.
Application Area:Fixable DNA stain
"Good DNA staining. Does not leak into viable cells after brief formaldehyde fixation."
Status:
ReviewerMember since: 2020
Organization: CU Anschutz
Simple and robust results.
Application Area:Live cell microscopy
"Simple and easy to use. Little to no background fluorescence (wide-field microscopy, LED illumination). Very clear and strong signal upon cell death."
Status:
ReviewerMember since: 2020
Organization: University of California, Irvine School of Medicine
Great consistent staining in fixed brain sections.
Application Area:Immunofluorescence: PFA fixed brain tissue sections
"Product works consistently well for immunofluorescence on PFA fixed brain sections. I used a dilution of 1:1000 in PBS 1x for 15 minutes and it works flawlessly every time."
Status:
ReviewerMember since: 2019
Organization: Shimane University, Medical Faculty (Japan)
Great results.
Application Area:Analyze apoptosis
"Solved an issue in our experiment that PI has overlapping excitation and emission wavelength with high concentration Adriamycin. We got desirable results after using DRAQ7 to label late-apoptotic cells."
Status:
ReviewerMember since: 2019
Organization: Enplas
Best for our experiments.
Application Area:Imaging cells in real time
"This dye has changed the way we can perform experiments so that they can be maintained for longer without losing cell viability. Together with Hoechst, it means we can estimate the proportion of dead cells in an entire population quickly, easily and over several time points."
Status:
ReviewerMember since: 2019
Organization: Srebrnjak Children's Hospital
DRAQ7 is now implemented in our standard SVF cells staining protocol.
Application Area:Detection of stromal vascular (SVF) cells isolated from lipoaspirate and microfragmented lipoaspirat
"DRAQ7 is a very stable and easy-to-use dye. We carefully titrated dye using fix/perm approach after the staining for 7 surface markers. After optimization of the staining protocol we achieved strong signal with an easy discrimination of nucleated cell from the cellular debris and fat particles. Samples were analysed on Navios flow cytometer."
Status:
ReviewerMember since: 2019
Organization: Uppsala University
Great result
Application Area:Analyze cell viability by FACS and confocal microscopy
"I found DRAQ7 with a very bright color specifically by using it in imaging. It is also very easy to use, just a drop and no long incubation is needed."
Status:
ReviewerMember since: 2019
Organization: CRCHUM
Great results.
Application Area:Cell death
"Very effective and easy to use. Been using the product for a very long time to identify cell death in treated tumor specimen."
Status:
ReviewerMember since: 2018
Organization: Hannover Medical School
Best choice for our application
Application Area:Three-dimensional live-cell imaging on implant
"We used DRAQ7 to monitor the cell death along implants over time. For this purpose we required a non-toxic and stable fluorescent dye to detect dead cells. DRAQ7 met all necessary requirements and worked also in the hydrogel. It was very easy to use with immediate results without optimization period. Some other advantages are that it works without UV light and is non-toxic to cells over time."
Status:
ReviewerMember since: 2018
Organization: Institute of Advanced Chemistry of Catalonia
Beautiful microscopy images.
Application Area:Labeling nucleous in cleared hidrogel-brain tissue (CLARITY)
"DRAQ7 has been used in Glioblastoma bystander cell therapy: improvements in treatment and insights into the therapy mechanisms. Is very easy to use. We got beautiful microscopy images you could find in our paper."
Status:
ReviewerMember since: 2018
Organization: Monash University
Reproducible result, basic component for quantitative measurement of dead cells.
Application Area:Quantitative measurement of dead cells in live cell imaging
"DRAQ7 works perfectly and always stain the nucleus of the dead cells. We use it each time to count the number of dead cells in live cell imaging. It works better than PI."
Status:
ReviewerMember since: 2018
Organization: Singapore-MIT Alliance for Research and Technology
Good product
Application Area:Analyze number of dead cells at each timepoint
"My colleagues and I could see a clear and bright signal even with low laser power. There was never an issue of poor dye penetration into 3D cellular aggregates, as would otherwise be observed with more common nuclear dyes, such as Hoechst . The quality of staining is usually consistent across experiments and among different colleagues. This follows that our results were also consistent across experiments. The dye was stable after repeated uses, and the signal intensity of DRAQ7 did not decrease with repeated use. Moreover, as it is simply added to cell culture media, it fits easily into any experimental design. I would recommend DRAQ7 to other researchers for its stability, fluorescence quality and its convenience of use. One suggestion for improvement would be to create versions in other fluorescence colors instead of just one which is presently APC."
Status:
ReviewerMember since: 2015
Organization: Charite University Medicine - Berlin
A useful far-red fluorescent dye for visualizing nuclei.
Application Area:Cell counting in cell culture
"DRAQ7 is a useful product for those wishing to visualize nuclei in the far-red. It was as simple to use as DAPI, but may have advantages over DAPI, as you don't need to use U.V. light to excite it."
Status:
ReviewerMember since: 2018
Organization: Duke-NUS Medical School, Singapore
Provided exactly the requested staining. Great results!
Application Area:Visualizing cell death in culture.
"We used DRAQ7 to visualize the formation of a necrotic core in fresh isolated Islets of Langerhans, which are prone to hypoxia. DRAQ7 was simply added to the medium to a final conc. of 1 microM. This compound appeared specific for staining the nuclei of necrotic cells only. It is easy in use, provides a fairly low background and stable regarding bleaching."
Status:
ReviewerMember since: 2017
Organization: Swansea University
Easy way to add valuable data to all our experiments
Application Area:Monitor cell viability
"Great value, straightforward way to assess cell viability through flow cytometry in all our experimental settings. Easily incorporated into the work flow to provide robust and easy to interpret data."
Status:
ReviewerMember since: 2016
Organization: Flow Cytometry Core Facility, Blizard Institute, Queen Mary London University of London, UK
Very bright photostable far red dye
Application Area:Flow Cytometry
"The dye was easily loaded into cells at 37C giving a high level of fluorescence at 760 nm making it easy to gate out dead cells on flow cytometric dot-plots. There was no apparent photo-bleaching of this far red dye either, quite impressive."
Status:
ReviewerMember since: 2017
Organization: Swansea University
Quality product, good value for money
Application Area:Immunometabolism
"Very easy to use, gives clear, consistent and reproducible results, which makes measuring cell death easy and reliable. Would strongly recommend this product for measuring apoptosis. Good value for money."
Status:
ReviewerMember since: 2017
Organization: University
Great product, will use it again
Application Area:Analyse impact of components on the fraction of compromised cells in drug delivery / drug uptake stu
"The product is easy to use, creates reliable results when comparing to other complementary techniques."
Status:
ReviewerMember since: 2016
Organization: INRA
DRAQ7 is an easy to use viability dye that allows monitoring of cell death over time.
Application Area:Cell viability
"DRAQ7 was used to measure cell death in complement-dependent-cytotoxicity experiment. Our project was to determine the ability of anti-Tn antibody to mediate death of Tn expressing in association with complement. Briefly, Tn expressing cells were incubated with anti-Tn antibody, in the presence of complement and DRAQ7 as a viability marker. Cell were incubated in Incucyte System (www.essenbioscience.com/en/products/incucyte?gclid=CObfsKOZjM8CFWMW0wodVw0JSQ) and the appearance of red fluorescence (staining of dead cells by DRAQ7) was monitored every 30 minutes. We were able to measure cell death over time by the use of DRAQ7. Fluorescence emission signal was high, and the results obtained were reproducible. Among all viability dye we have tested, it was the only one that emits strong signal after several hours of incubation. "
Status:
ReviewerMember since: 2016
Organization: CHU de Quebec - Université Laval, Canada
Superior to propidium iodide.
Application Area:cell viability, FACS
"DRAQ7, a cell impermeant DNA stain, is great to assess cell viability using FACS: low autofluorescence in the infrared, ease to combine it with other fluorophores in the visible range. Much better than propidium iodide on the last account."
Status:
ReviewerMember since: 2016
Organization: University of Edinburgh
Great for imaging cell death as well as FACS
Application Area:Small molecule cell death screens
"Draq7 offers a great means in which to image cell death in real time. Although the product is commonly used as a FACS marker for cell death, I have found great utility as a way to real time mark cell death using Incucyte. Con is price is slightly higher than competitor products used previously, but Draq7 is still my go to marker for cell death"
Manufacturer's Response“Thank you for your review of DRAQ7. For higher throughput applications we offer a larger pack size (Code: DR710hc) reducing cost per assay by 45%”
Status:
ReviewerMember since: 2011
Organization: Imagen biotech
Application Area:High Content Screening
"This has enabled us to do experiments that were not otherwise possible before. Not only is it a much cleaner signal than other viability dyes, but the fact that it is non-toxic has enabled us to do time courses with the same samples. We have performed 3D spheroid experiments over a 4week period, reading plates every 72hours with zero toxicity! It therefore saves us large amounts of money that would have been spent on ending experiments at each time point using toxic dyes. Highly recommended! "
Status:
ReviewerMember since: 2015
Organization: HOSPITAL INFANTIL DE MÉXICO FEDERICO GÓMEZ
"We used to stain nuclei DRAQ7 cardiac tissue of rats that developed cardiac hypertrophy embedded in paraffin. The reagent was quite stable, not gender line, besides having a very long life."
Manufacturer's Response"Alejandra
We sincerely apologise if our distribution partners in Mexico are not performing to our high standards. If there is an issue with the local agents it is always possible to order direct from BioStatus for immediate delivery and our 24h response time for tech support."
Status:
ReviewerMember since: 2015
Organization: GENyO Centre, FPS
Application Area:Viability dye in whole blood flow cytometry
"We have used DRAQ7 as a convenient viability dye in whole blood Lyse-no Wash flow cytometry protocols, included in a 8-color panel. The staining we got was excellent and no major compensation issues arrived. We will definitively keep using this dye in further projects. "
Status:
ReviewerMember since: 2015
Organization: Tottori University
"The greatest characteristic of DRAQ7 is Ease of Use. Short incubation time (~10 min) and excitation by near-infrared wavelength allow us to sort and eliminate dead cells in flow cytometric analysis easier. This dye also has good performance considering its low price."
Status:
ReviewerMember since: 2014
Organization: Tohoku University School of Medicine
Application Area:Immunocytochemistry
"This product is easy-to-use, with very low photobleaching during confocal microscopic examination."
Status:
ReviewerMember since: 2014
Organization: Bar-Ilan University
Application Area:Cell Health (U-251 cells)
"This product is user-friendly and does not require much effort to use. It gives good results and is clear. It can be used for a time acceptable. In addition, the company's service was superior in terms of response and availability. I am very pleased and I know I will contact the company again when I need to. "
Status:
ReviewerMember since: 2012
Organization: Memorial Sloan-Kettering Cancer Center
Application Area:Live cell screening
"Draq7 is a great dye for labelling cells and is very easy to use. Using this dye we have been able to do live cell screening of cells, as it is non-toxic to cells. However, overall the cost is a bit expensive for screening."
DRAQ7 fluoresces in the far-red – with the same spectral properties that have made DRAQ5™ a reagent of choice in HCS.
DRAQ7™ only labels membrane-compromised dead, dying and apoptotic cells and gives clear discrimination between these and healthy cells in high content cytotoxicity and viability assays and in flow cytometry.
The far-red emission, longer wavelength than TOTO-3, permits better combination with other fluorescent probes. It is spectrally compatible with the commonly used orange/red mitochondrial health probes, and UV-excited vital dyes.
DRAQ7 can be used in RNAi knock-down and long-term, dynamic viability assays to report in vitro cytotoxicity and apoptosis.
Also, DRAQ7 efficiently counterstains fixed cell and tissue preparations. It offers the same differential nucl:cyto staining for dual compartment segmentation in translocation tracking and cell morphometrics for in vitro toxicology, that has made DRAQ5™ a favourite of HCS assay developers.
DRAQ7 is supplied as a convenient aqueous, ready-to-use solution. The 1 ml pack size (GBP 140.00 / ca. USD 220.00) is sufficient for at least 200 flow cytometry tests, 300-600 slides and up to 10,000 1536-MTP wells.