DRAQ7 by Biostatus Limited

"Easy to use, reproducible and consistent results."

"Simple and easy to use. Little to no background fluorescence (wide-field microscopy, LED illumination). Very clear and strong signal upon cell death."

"Product works consistently well for immunofluorescence on PFA fixed brain sections. I used a dilution of 1:1000 in PBS 1x for 15 minutes and it works flawlessly every time."

"Solved an issue in our experiment that PI has overlapping excitation and emission wavelength with high concentration Adriamycin. We got desirable results after using DRAQ7 to label late-apoptotic cells."

"This dye has changed the way we can perform experiments so that they can be maintained for longer without losing cell viability. Together with Hoechst, it means we can estimate the proportion of dead cells in an entire population quickly, easily and over several time points."

"DRAQ7 is a very stable and easy-to-use dye. We carefully titrated dye using fix/perm approach after the staining for 7 surface markers. After optimization of the staining protocol we achieved strong signal with an easy discrimination of nucleated cell from the cellular debris and fat particles. Samples were analysed on Navios flow cytometer."

"I found DRAQ7 with a very bright color specifically by using it in imaging. It is also very easy to use, just a drop and no long incubation is needed."

"Very effective and easy to use. Been using the product for a very long time to identify cell death in treated tumor specimen."

"We used DRAQ7 to monitor the cell death along implants over time. For this purpose we required a non-toxic and stable fluorescent dye to detect dead cells. DRAQ7 met all necessary requirements and worked also in the hydrogel. It was very easy to use with immediate results without optimization period. Some other advantages are that it works without UV light and is non-toxic to cells over time."

"DRAQ7 has been used in Glioblastoma bystander cell therapy: improvements in treatment and insights into the therapy mechanisms. Is very easy to use. We got beautiful microscopy images you could find in our paper."

"DRAQ7 works perfectly and always stain the nucleus of the dead cells. We use it each time to count the number of dead cells in live cell imaging. It works better than PI."

"My colleagues and I could see a clear and bright signal even with low laser power. There was never an issue of poor dye penetration into 3D cellular aggregates, as would otherwise be observed with more common nuclear dyes, such as Hoechst . The quality of staining is usually consistent across experiments and among different colleagues. This follows that our results were also consistent across experiments. The dye was stable after repeated uses, and the signal intensity of DRAQ7 did not decrease with repeated use. Moreover, as it is simply added to cell culture media, it fits easily into any experimental design. I would recommend DRAQ7 to other researchers for its stability, fluorescence quality and its convenience of use. One suggestion for improvement would be to create versions in other fluorescence colors instead of just one which is presently APC."

"DRAQ7 is a useful product for those wishing to visualize nuclei in the far-red. It was as simple to use as DAPI, but may have advantages over DAPI, as you don't need to use U.V. light to excite it."

"We used DRAQ7 to visualize the formation of a necrotic core in fresh isolated Islets of Langerhans, which are prone to hypoxia. DRAQ7 was simply added to the medium to a final conc. of 1 microM. This compound appeared specific for staining the nuclei of necrotic cells only. It is easy in use, provides a fairly low background and stable regarding bleaching."

"Great value, straightforward way to assess cell viability through flow cytometry in all our experimental settings. Easily incorporated into the work flow to provide robust and easy to interpret data."

"The dye was easily loaded into cells at 37C giving a high level of fluorescence at 760 nm making it easy to gate out dead cells on flow cytometric dot-plots. There was no apparent photo-bleaching of this far red dye either, quite impressive."

"Very easy to use, gives clear, consistent and reproducible results, which makes measuring cell death easy and reliable. Would strongly recommend this product for measuring apoptosis. Good value for money."

"Good and useful product to stain nuclei in our experiments"

"The product is easy to use, creates reliable results when comparing to other complementary techniques."

"DRAQ7 was used to measure cell death in complement-dependent-cytotoxicity experiment. Our project was to determine the ability of anti-Tn antibody to mediate death of Tn expressing in association with complement. Briefly, Tn expressing cells were incubated with anti-Tn antibody, in the presence of complement and DRAQ7 as a viability marker. Cell were incubated in Incucyte System (www.essenbioscience.com/en/products/incucyte?gclid=CObfsKOZjM8CFWMW0wodVw0JSQ) and the appearance of red fluorescence (staining of dead cells by DRAQ7) was monitored every 30 minutes. We were able to measure cell death over time by the use of DRAQ7. Fluorescence emission signal was high, and the results obtained were reproducible. Among all viability dye we have tested, it was the only one that emits strong signal after several hours of incubation. "

"DRAQ7, a cell impermeant DNA stain, is great to assess cell viability using FACS: low autofluorescence in the infrared, ease to combine it with other fluorophores in the visible range. Much better than propidium iodide on the last account."

"Draq7 offers a great means in which to image cell death in real time. Although the product is commonly used as a FACS marker for cell death, I have found great utility as a way to real time mark cell death using Incucyte. Con is price is slightly higher than competitor products used previously, but Draq7 is still my go to marker for cell death"

"Very easy to use product for live cell analysis. Quick and efficient."

"This has enabled us to do experiments that were not otherwise possible before. Not only is it a much cleaner signal than other viability dyes, but the fact that it is non-toxic has enabled us to do time courses with the same samples. We have performed 3D spheroid experiments over a 4week period, reading plates every 72hours with zero toxicity! It therefore saves us large amounts of money that would have been spent on ending experiments at each time point using toxic dyes. Highly recommended! "

"We used to stain nuclei DRAQ7 cardiac tissue of rats that developed cardiac hypertrophy embedded in paraffin. The reagent was quite stable, not gender line, besides having a very long life."

"We have used DRAQ7 as a convenient viability dye in whole blood Lyse-no Wash flow cytometry protocols, included in a 8-color panel. The staining we got was excellent and no major compensation issues arrived. We will definitively keep using this dye in further projects. "

"The greatest characteristic of DRAQ7 is Ease of Use. Short incubation time (~10 min) and excitation by near-infrared wavelength allow us to sort and eliminate dead cells in flow cytometric analysis easier. This dye also has good performance considering its low price."

"This product is easy-to-use, with very low photobleaching during confocal microscopic examination."

"It is very easy to use. DRAQ is my routine far-red DNA dye."

"This product is user-friendly and does not require much effort to use. It gives good results and is clear. It can be used for a time acceptable. In addition, the company's service was superior in terms of response and availability. I am very pleased and I know I will contact the company again when I need to. "

"Draq7 is a great dye for labelling cells and is very easy to use. Using this dye we have been able to do live cell screening of cells, as it is non-toxic to cells. However, overall the cost is a bit expensive for screening."