Status:
ReviewerMember since: 2024
Organization: Sapienza University (Rome, Italy)
Very simple use to detect the nuclei and the signal is generally bright.
Application Area: To visualize nuclei for basic research
"It is easy to use and the staining is generally bright. In my case it was helpful since it was not possible to detect the fluorescence released by other compounds used to visualize nuclei such as dapi or hoechst 33342."
Status:
ReviewerMember since: 2024
Organization: IRCCS INMI Spallanzani
Great results!
Application Area:Confocal microsopy
"The product is highly effective for confocal microscopy use and easy to use. We could achieve high-quality and reproducible results. Quality meets the value of money, also considering the after-sales care."
Status:
ReviewerMember since: 2023
Organization: Hertie Institute for Clinical Brain Research
Great nuclear counterstain
Application Area:Nuclear counterstaining in fluorescence microscopy
"DRAQ5 is a bright and easy-to-use nuclear counterstain in the red part of the spectrum. It doesn't require washing after incubation and hence can save you a couple of minutes in the lab."
Status:
ReviewerMember since: 2023
Organization: plateforme CYM - UMS IPSIT
A bacterial DNA DRAQ5 was added to differentiate them from the background.
Application Area:labeling bacterial viability for flow cytometry and confocal microcopy analysis
"We ordered the product from Thermo Fisher. It is very easy to use in an effective and economical concentration range (after titration). Its use on fixed bacteria is a success. The protocol we have developed is the subject of a technical note: A flow cytometry method for safe detection of bacterial viability (DOI: 10.1002/cyto.a.24794)"
Status:
ReviewerMember since: 2023
Organization: Università del Piemonte Orientale
Great results
Application Area:Cell cycle analysis of leukemia cells
"We have obtained excellent results staining acute myeloid leukemia cells for flow cytometric analysis of the cell cycle."
Status:
ReviewerMember since: 2023
Organization: University of Patras
Great and reliable results, easy to use, recommended in IF experiments
Application Area:analyze tissue samples and cells
"DRAQ5 is easy to use and provides reliable and consistent staining, when the datasheet information is followed. High-quality results are achieved in confocal microscope in immunofluorescence experiments using cells or tissues."
Status:
ReviewerMember since: 2023
Organization: MGH
Great result
Application Area:Nuclear Staining
"Easy to use. Just followed the manufacture's protocol and got great microscopy images. To lower the autofluorescence which we observed with Hoechst33342 on certain materials, we chose DRAQ5 and could get clear nuclear staining images."
Status:
ReviewerMember since: 2023
Organization: INPer, Mexico
It is easy to use and reliable as an internal control
Application Area:In cell wester blot (in neural tube coronal slices)
"The product is ideal as an internal control for immunoflorometryc quantification using immunofluorescence (infrared range). Results are reproduced consistently."
Status:
ReviewerMember since: 2022
Organization: Semmelweis University
The results were good, but DraQ5 was too expensive, its utilization was limited.
Application Area:detect the cell nuclei in confocal fluorescent microscopy
"The product worked well. Our main problem with these stains is that they cannot be used in any microscope, e.g. Draq5 can be used in a confocal laser microscope, but cannot be used in another, simpler microscope, so we have to purchase different stains for different microscopes. In addition, DraQ5 is more expensive than other similar stains."
Manufacturer's ResponseThank you for your review of DRAQ5. You are right that few epifluorescence microscopes have far-red detection capabilities. You may wish to explore the use of CyTRAK Orange which is excited optimally at 510 nm (488 ex. compatible) and with peak emission at 615 nm (“rhodamine" channel). We do appreciate your concern about pricing.. sadly we have no legal control over end-user pricing by our channel partners. You might, however, consider purchasing direct from BioStatus for the keenest price, fastest service and our expert technical support.
Status:
ReviewerMember since: 2022
Organization: University La Sapienza of Roma
Great results!
Application Area:Analyze nucleus in confocal microscopy
"This product is very simple to use and gives high quality result. It also guarantees reproducibility of experiments. I used it for nucleus staining in confocal microscopy at 1:1000 concentration."
Status:
ReviewerMember since: 2022
Organization: Center for Neuroscience and Cell Biology (CNC)
An easy-to-use DNA dye suitable for tissue clearing of mouse brain samples
Application Area:Fluorescence microscopy counterstain for tissue clearing samples
"The DraQ5 fluorescent probe is supplied as a water-soluble concentrated solution (0.5mM or 5mM) that can be diluted depending on the desired application. Given its high affinity for double-stranded DNA, DraQ5 can be used as a nuclear counterstain in immunohistochemistry studies upon short incubation periods of time, as an alternative to blue-fluorescent DNA-binding probes. However, the broad excitation and emission peaks must be taken into account if a combination with other fluorophores is desired. In our experience, it is also compatible with aqueous-based tissue clearing techniques, rendering a uniform labelling across entire mouse brain hemispheres (55-days-old) that could be imaged by light-sheet fluorescence microscopy. To achieve this, a simple incubation at 37⁰C/60rpm for 12h with a DraQ5 diluted solution (2.5uM) and a 12-hour washing step at 4⁰C to remove excess were incorporated in the tissue clearing procedure."
Status:
ReviewerMember since: 2022
Organization: Pasteur institute of Iran
Great result
Application Area:Nuclear staining
"The product was easy to use. We achieved high-quality results. We took excellent photos with the confocal microscope. The results were reproducible"
Status:
ReviewerMember since: 2022
Organization: cas
It is ok
Application Area:Cancer imaging
"The effect is ok, but not very stable"
Manufacturer's ResponseDear Zi, It would be helpful to understand what the issue was in more detail. If staining is not stable over time that can be the case in live cells, where after several hours a re-distribution can occur in some cell types, mostly towards lipophilic structures in the cytoplasm (Martin, 2005). If the staining is not stable in fixed cells then this may be due to washing post-staining, too low concentration, or not removing surfactants used in prior cell permeabilisation procedures.
Status:
ReviewerMember since: 2022
Organization: Charles University
Easy to use. Nice results
Application Area:Detection of nuclei in CUBIC cleared samples for light sheet microscopy
"Easy to use. Nice results"
Manufacturer's ResponseThank you so much for the product review, Martin. We value this feedback! We would like to understand how we can do better at after-sales service as we have no record of a direct enquiry, but understand that there maybe at-arms-length improvements to undertake.
Status:
ReviewerMember since: 2022
Organization: Norwegian University of Science and Technology
Excellent nuclear stain. Easy to use. Works well with other common stains
Application Area:Cell Staining (nuclei/DNA)
"DRAQ5 is simple to use since a washing step is not required. It provides a strong signal and good visualization of cell nuclei when used in confocal laser scanning microscopy (CLSM). Its red fluorescent spectrum makes it a suitable stain to work with in conjunction with other common blue and green stains as signal overlap can be avoided."
Status:
ReviewerMember since: 2022
Organization: Politecnico di Milano, Dip Chimica Mat e Ing. Chimica G.Natta
Great product and highly reliable
Application Area:Intravital fluorescence microscopy in immune reaction observations
"The product is perfect to be used in in vivo applications. It allowed cell nuclei segmentation from intravital fluorescence images. OK both in mammalians and chicks."
Status:
ReviewerMember since: 2019
Organization: La Trobe University
Far red cell nucleus stain
Application Area:Cell colocalization analysis
"DRAQ5 is generally easy to use. The spectra are in long wavelength that can be paired with many other fluorophores and thus our choice of nucleus dye for confocal imaging. However, the performance is not very stable, sometime it can be very dim and unspecific staining outside the nucleus can be seen. The product (from Thermo Fisher) is very expensive. It will be good if the price can be more reasonable."
Manufacturer's ResponseDear Yuning, thank you for detailed review of DRAQ5. Without seeing examples of the issues you raise it is difficult to give further technical advice. The occasional cytoplasmic staining you describe is often a symptom of understaining or washout due to residual surfactant left over from a prior washing step. Regarding cost, there are a number of other carefully selected suppliers you can choose from and we can advise on the most competitive of these or alternatively order direct from us, the source!
Status:
ReviewerMember since: 2021
Organization: University of Wyoming
Compared with other nuclear dyes, this is one of the best, at least, in our hands.
Application Area:Budding yeast nuclear staining of live cells
"DRAQ5 is very easy to use. You resuspend cells in PBS, stain cells in dark for 1-3 minutes, then you can image cells directly (no rinsing is needed). The product is kind of expensive, but it is worth it. The dye can be excited by either 488 or 639 nm laser, but emits in far red, so it won't interfere with GFP."
Status:
ReviewerMember since: 2021
Organization: The Scripps Research Institute, Flotida
Excellent reproducible results.
Application Area:Fluorescently paint cell nucleus
"The DRAQ5 reagent is very easy to use. I have used it together with DAPI for counterstaining the cellular nuclei in various cell types like SHSY-5Y, SKN-SH, U2OS and HeLa. The reagent is robust and the staining is perfectly reproducible. I had used the probe in a variety of application like Fluorescent Microscopy, FISH and combined FISH with ICC. The probe lifetime is commendable even after multiple exposures. You can find images with application of DRAQ5 in my publication here - Chanda, K., Jana, N.R. & Mukhopadhyay, D. Receptor tyrosine kinase ROR1 ameliorates Aβ1–42 induced cytoskeletal instability and is regulated by the miR146a-NEAT1 nexus in Alzheimer’s disease. Sci Rep 11, 19254 (2021). https://doi.org/10.1038/s41598-021-98882-0"
Status:
ReviewerMember since: 2021
Organization: University of Toronto
Great product!
Application Area:Imaging of engineered tissues
"This product is easy to use and gives us a nice consistent nuclear signal in our engineered tissue models. Since it emits in far-red, it's great for multiplexing with common fluorophores that emit in green/blue/red."
Status:
ReviewerMember since: 2021
Organization: Harvard University
It is one of the best probes I have ever worked on!
Application Area:Cell counting in high-content compound screening
"We have run high-content compound screening and used this product for cell counting. It works in every experiment: the staining is simple, rapid and efficient, the signal is strong, it does not interfere with other probes."
Status:
ReviewerMember since: 2021
Organization: The University of Tokyo
A necessary tool for immunocytochemistry.
Application Area:Immunocytochemical analysis
"This product is very easy to use and can stain nuclei with high sensitivity."
Status:
ReviewerMember since: 2021
Organization: VCU
Easy & quick
Application Area:In Cell Western - protein normalization
"Had no issues with the DRAQ5 and it produced reliable and repeatable staining of DNA."
Status:
ReviewerMember since: 2021
Organization: Università Federico II Napoli
Great results.
Application Area:Cell biology
"It’s a very good product for our application. It is very easy to use."
Manufacturer's ResponseThank you for your opinion Emanuela! It’s great to have this positive feedback!
Status:
ReviewerMember since: 2021
Organization: erasmus mc
Blue cells.
Application Area:Identification of single nucleated cells
"Easy to use, very potent, just add to your cells and their nuclei will turn blue. Easy peasy lemon squeezy."
Manufacturer's ResponseThanks Héctor, for this entertaining review of DRAQ5! You make a good point too: it is possible to view DRAQ5-positive cells with transmission light microscopy - the nuclei are indeed deep blue (as well as far-red fluorescent)! It’s a convenient way to test for functionality and sample quality (for example prior to FACS) and might even allow for a new breed of assays.
Status:
ReviewerMember since: 2021
Organization: University of Nebraska Medical Center
The product is of high quality and easy to use. I would recommend it to everyone.
Application Area:Cell staining
"The product is reliable and works out great. It gives me consistent staining results with high quality. It stains nuclei easily and produces clear images suitable for publication."
Status:
ReviewerMember since: 2021
Organization: University of Cambridge
The most effective nuclei stain for live cells in our cell type of interest.
Application Area:FACS analysis, IF microscopy
"The product was highly effective in helping us identify the nuclei of both live/dead cells. I will be purchasing this product again and believe it to be highly reliable and effective."
Status:
ReviewerMember since: 2021
Organization: POLITECNICO DI MILANO
Very good.
Application Area:Fluorescence imaging
"I am very satisfied with the product and I often recommend it to other researchers, especially due to its excitation/emission spectrum. In fact, it is very suitable for those who work with autofluorescent scaffolds/biomaterials. It is easy to use, it is a vital dye and marks the cell nucleus well, although sometimes it provides a slightly dirty staining."
Status:
ReviewerMember since: 2021
Organization: Universidad Autónoma de Madrid
It is a very good option for labelling cell nuclei.
Application Area:Immunohistochemistry
"This product is very easy to use and always offer reproducible results."
Status:
ReviewerMember since: 2021
Organization: University of Turin
Great product, only a little bit pricy!
Application Area:Analyze endosome position respect to the nucleus in mammalian cells
"The DRAQ5 is an easy usable marker to stain nucleus. I used it in mammalian cells. It is a really good product, maybe a little expensive."
Manufacturer's ResponseDear Giulia, thank you for your valued feedback and independent review. If you have a concern over local pricing we can assist by supplying direct. Also, the small cost of a proven, reliable nuclear counterstain that can be used for 1000-2000 slides should be seen against the significant cost of multiple fluorescently-tagged monoclonal antibodies used alongside it and that its far-red emission enables.
Status:
ReviewerMember since: 2021
Organization: Italian Institute of Technology
Great results.
Application Area:Cell nuclei staining
"Good cell staining to characterize the model by means of confocal microscopy."
Status:
ReviewerMember since: 2020
Organization: Assitance Publique Hôpitaux de Marseille AP-HM
Great results.
Application Area:Characterization of cell therapy
"Easy to use. Reproducible. Slight improvement would be to have an already diluted form."
Manufacturer's ResponseDear Pauline, thank you for the generous review and the constructive suggestion. We have produced a diluted presentation of DRAQ5 (at 500 µM rather than 5 mM). The product code is DR05500 and, having reviewed your protocol, would perhaps allow you to skip the pre-dilution step entirely e.g. 4 µl into a final volume of 100 µl would give a final concentration of 20 µM. Perhaps this might assist you better?”
Status:
ReviewerMember since: 2020
Organization: First affilated hospital of Shantou University Medical College
Stable results.
Application Area:Enucleation of red blood cells
"The product is easy to use and the procedure is not complicated. We used this stain to evaluate the enucleation rate of cultured red blood cell differentiated from CD34+ cells."
Status:
ReviewerMember since: 2020
Organization: NYU Langone Health
Easy-to-use DRAQ5 with excellent results.
Application Area:Immunofluorescent analysis of fixed mouse brain tissues
"DRAQ5 is an easy-to-use product that is ready straight from the bottle and just have to be diluted to a working concentration. As with almost all dyes and antibodies for immunofluorescence, it has to be tested for the ideal concentration but the product came with suggested working solution concentrations that proved to be very useful as I had to do just a couple of tests to establish the working conditions. The dye is very bright and long-lasting, and the reagent itself lasts for long time at 4 degrees C."
Status:
ReviewerMember since: 2020
Organization: UNIFESP
Great results and easy to be use.
Application Area:Immunology
"The product is easy to use and labeled my cells very well. I recommend using it in cell assays such as image flow cytometry."
Status:
ReviewerMember since: 2020
Organization: University of Michigan
DRAQ5 is my favorite!
Application Area:Microscopy - DNA stain
"Love DRAQ5 for simple and bright nuclear staining of live or fixed cells for confocal microscopy. Minimal crossover in typical filter sets."
Status:
ReviewerMember since: 2020
Organization: University of Campinas
DRAQ5 is bright and stable
Application Area:nanoparticles and cells interactions
"I've been using DRAQ5 for some time, I like it very much, it's efficient (bright and quite stable), but I wish it was cheaper."
Manufacturer's ResponseDear Marcelo, Thank you for taking the time to review DRAQ5. We have no control over end-user prices but it is possible to purchase direct from BioStatus, where we are transparent about our pricing - published on our website in GBP, USD and EUR. However, we will investigate this directly with you to find a better, less expensive way for you and other scientists in Brazil to get the far-red benefits of DRAQ5!
Status:
ReviewerMember since: 2020
Organization: Eötvös Loránd University
Easy to use, reliable nuclear stain.
Application Area:Nuclear stain for cell counting
"Easy to use, reliable nuclear stain. Can be used for the analysis of cell counts on primary human monocytes, monocyte-derived macrophages and dendritic cells. One vial is enough for a long time as it is used in a 1000x dilution."
Status:
ReviewerMember since: 2020
Organization: Universidad de Buenos Aires
Great results
Application Area:Immunohistochemistry
"Draq5 is very easy to use and gives reproducible resutls. In each immunostaining that I used Draq5 always worked fine."
Status:
ReviewerMember since: 2020
Organization: ESB
The innovative approach gave positive results.
Application Area:Studying microbial distribution in porous materials
"We have used DRAQ5 to follow the bacterial distribution on wooden material. Our results are cited in (doi:10.3390/antibiotics9050225) and (doi:10.1007/s00107-018-1294-z)."
Status:
ReviewerMember since: 2020
Organization: Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice,Slovak Repub
Decent nuclear staining in far red channel.
Application Area:Nuclear staining
"If there is the need to visualize fixed nuclei by confocal microscopy in the far red, then Draq5 is the stain of choice. Of course it is not as bright as DAPI and probably the "gain" and "laser power" should be considerably increased. Also some leakage of staining in cytoplasm could be observed, but still it is very reliable for staining. For the best results, dilution 1:500 in 0.1M PBS (alternatively with 0.3% Triton X) should be used."
Status:
ReviewerMember since: 2020
Organization: EnGeneIC Pty Ltd
Easy-to-use product.
Application Area:Flow cytometry live/dead staining
"The product is easy to use and provides clear live/dead staining of multiple sample types."
Status:
ReviewerMember since: 2018
Organization: University of Barcelona, Bellvitge University Hospital, IDIBELL, CIBERNED
Good results; recommended in the current histological practice.
Application Area:Neuropathology
"Good nuclear marker for single- and double-labelling immunofluorescence and confocal microscopy on frozen and paraffin-embedded sections. Staining disappears in sections pre-treatment with formic acid (as in prion diseases). Sometimes, non-specific staining of other structures."
Manufacturer's ResponseThank you very much for the valuable feedback and product review on DRAQ5. The observation about the use of formic acid is useful to know. Perhaps it may be worthwhile to attempt staining with DRAQ5 as the final step after all other steps are complete, including the formic acid treatment. It is unusual to see staining of other structures outside of the nucleus with DRAQ5 but may be a consequence of the treatments of the sample. DRAQ5 does have, like all cell permeant drug-like small molecules, a propensity for binding to lipophilic structures in addition to the specific target, in its case dsDNA, but this is usually only seen in cases of understaining, paradoxically. We are always ready to answer these questions through our tech support service as they occur in real-time in the bench experiments.
Status:
ReviewerMember since: 2019
Organization: La Trobe University
Nucleus stain doing its job
Application Area:Cell imaging co-localization analysis
"We used DRAQ5 because of its far-red fluorescence and suitability for live/fixed-cell imaging. In general, it suits our needs as we can't find a commercial alternative out there. It is easy to use, but sometimes the fluorescence can be very dim and needs some adjustment to make it visible. We bought it from ThermoFisher/Invitrogen so it is quite expensive."
Status:
ReviewerMember since: 2019
Organization: Italian institute of Technology Napoli (CRIB)
Best results.
Application Area:Cell culture studies
"We have been using the product for a while and will now use it for most of our biological studies. The reproducibility was good. I have already recommended the product to my colleagues. Perfect value for money."
Status:
ReviewerMember since: 2019
Organization: Albert Einstein College of Medicine
Easy stain.
Application Area:Evaluation of erythroid cell enucleation
"DRAQ5 is easy to use and gives reproducible results. It can be added to the cells at the last moment notably after performing antibody staining and does not require washing the cells prior analysis."
Status:
ReviewerMember since: 2019
Organization: CNRS
Avoid when studying DNA replication /repair /transcription /organization in cellulo.
Application Area:Supravital DNA fluorescent dye
"DRAQ5 is fine unless you want to study DNA replication, repair, transcription, etc in living cells. Why? Because, like other DNA intercalators, DRAQ5 perturbs the DNA macromolecule and will evict histones and a variety of DNA binding proteins."
Manufacturer's ResponseDear Pierre-Olivier, Thank you for your review of DRAQ5. It is indeed a well-understood phenomenon that all cell-permeant DNA-binding probes will have some impact on cell behaviour. Accordingly, we alert users to this on the front of the product’s technical datasheet. Nonetheless, DRAQ5 has been widely used for live-cell end-point assays and as a means of identifying the nucleated cells in flow cytometry experiments on complex sample matrices such as bone marrow, besides its popularity as a standard IF counterstain.
Status:
ReviewerMember since: 2019
Organization: Research Center Juelich GmbH
Great results!
Application Area:Nuclei staining
"Excellent and reliable staining of the nuclei! The only drawback is that sometimes it stains more than just the nuclei. It helped with the cell counting program."
Manufacturer's ResponseThank you for your review of DRAQ5. DRAQ5 does have a useful weak secondary staining of the cytoplasm which HCS labs use to segment the cell envelope, by increasing the gain. An unwanted inversion staining (“hollow” nuclei) is a consequence of too low a concentration of DRAQ5. We strongly recommend 5 uM for fixed cells and 5-10 uM for live cells. We would be very happy to discuss this and troubleshoot the issue to try to resolve it.
Status:
ReviewerMember since: 2015
Organization: Jacobs University Bremen
Standard nuclei/DNA counterstain in our lab for more than 15 years!
Application Area:DNA counterstain for immunofluorescence studies
"Super easy to use, just add it to your secondary antibody solution in immunofluorescence studies (cells and tissues), and you get reproducible stainings of your nuclei with excitation/emission wavelenghts that do not interfere with other common flurophores used in multiple immunofluorescence stainings."
Status:
ReviewerMember since: 2019
Organization: Institute for Research and Innovation in Health Sciences (i3S)
Great results, very useful for my experiments.
Application Area:Stain bacteria
"This product was effective for my experiments. I successefully stain my bacteria for my live/dead assays. I obtained good results and I consider that this product is easy to use."
Status:
ReviewerMember since: 2019
Organization: Fondazione IRCCS Istituto Nazionale dei Tumori di Milano
Good and reproducible results.
Application Area:Fluorescence microscopy
"The product is very easy to use, not too expensive. it does not need specific after sale care. The product allows for reproducible results."
Status:
ReviewerMember since: 2019
Organization: Pasteur Institute
The dye worked great in our experiment.
Application Area:Drug discovery and screening
"The Draq5 dye worked excellently in our assay. We used it for an image based assay for screening our drug candudates."
Status:
ReviewerMember since: 2019
Organization: Instituto de Tecnología Química
It is a good product.
Application Area:This product has been used in flow cytometry assays
"This product has been used in flow cytometry assays. This product is characterized by easy operation. It suffices to follow the manufacturer's guidelines. The results are reproducible. The product stands out further on account of its functionality and quality/price ratio."
Status:
ReviewerMember since: 2019
Organization: Institute of Innate Immunity - BMZ - University Hospital of Bonn
Very easy to use and works well.
Application Area:Cell nucleus staining
"Very easy to use and works well. Gives reliable and reproducible results."
Status:
ReviewerMember since: 2018
Organization: Cleveland Clinic Lerner Research Institute
Easy to use and yields consistent results.
Application Area:Cellular viability staining for flow cytometry. Used in concert with propidium iodide to differentia
"This product allowed us to effectively differentiate live and dead cells and debris when used in concert with propidium iodide for flow cytometric analysis. DRAQ5 can be diluted directly in FACSFlow when resuspending cells and offers a short incubation time. We were able to use this DRAQ5 when analyzing viability in several cell treatment groups and the results obtained matched the expected results."
Status:
ReviewerMember since: 2018
Organization: Radboud University
Good, reliable, easy to use product.
Application Area:In Cell Western analysis
"DRAQ5 is a versatile and easy to use DNA stain. We use it for high/medium throughput gene perturbation screens in mammalian cells."
Status:
ReviewerMember since: 2018
Organization: Institute of tropical medicine
Good results.
Application Area:Analyze efficacy of novel compounds
"The product was easy to use and the results were reproducible. I would recommend the product."
Status:
ReviewerMember since: 2018
Organization: UNICAMP
Can't live without it
Application Area:Analyze Nanoparticles-Cell interaction
"It's a very good product! I use it for fluorescence and confocal microscopy and i really like it"
Manufacturer's ResponseDear Allan, thank you for your positive review of DRAQ5. We are keen to understand how we can improve our after-sales service and would appreciate your feedback.
Status:
ReviewerMember since: 2018
Organization: Anglia Ruskin University
Consistent results, easy to use, what's not to love?
Application Area:Use as measure of cell viability and to determine cell numbers
"The DRAQ5 is essential for our In-Cell ELISA assays to measure the cell numbers and viability. It is super easy to use and lasts extremely long. It consistently delivers great results and we have never run into any problems with this reagent."
Status:
ReviewerMember since: 2018
Organization: Sapienza University of Rome
Great results, I would recommend it.
Application Area:DNA marker on mouse cerebellar slices
"It's an easy-to-use product of high quality. The results are good and reproducible."
Status:
ReviewerMember since: 2018
Organization: university of Turin
Very good results, absolutely worth trying.
Application Area:Immunofluorescence on fixed brain tissue
"This product is very easy to use and gives excellent staining, highly reproducible among distinct experiments. The results are comparable to the commonly used DAPI. Very good results also after antigen retrieval protocols. Absolutely worth trying."
Status:
ReviewerMember since: 2018
Organization: Fox Chase Cancer Center
The quality of the results obtained with this product are sub-optimal. Not so great!
Application Area:Analyzed nuclei in Drosophila ovaries using Draq5
"The product itself is easy to use but the results are not reproducible every time. Would love if the product quality is improved because overall it is a good reagent to have!"
Manufacturer's ResponseThe initial review posting elicited an immediate technical support response. After successful testing of both fresh DRAQ5 and DRAQfx (for comparison) in the customer’s lab the issue was isolated to the presence of 0.3% Triton X-100 during the final counterstaining step. This was remedied by an intermediate wash with PBS. This learning will be reflected in our products’ technical data sheets for fixed cell immunofluorescence protocols.
Status:
ReviewerMember since: 2018
Organization: Candiolo Cancer Institute
Good product
Application Area:Highlight nuclei in mitosis imaging
"The product is very easy to use and I followed the manufacturer's instructions. It works very well and does staind the nuclei. Unfortunately 6 months after opening the quality got lower since precipitates were shown in my images. I kept it at 4° as requested. It would be useful to be able to buy smaller aliquots for labs only using it to stained fixed cells."
Manufacturer's ResponseThe presence of precipitates in the vial is most unusual and could only be a result of contamination or freezing (accidental, most usually). We would like to have explored this with you at the time you saw this. Your comment about smaller quantities for fixed cell staining is well made - we have developed a product exactly of this nature - please see DRAQfx which is specifically for fixed cells, provided in a convenient dropper bottle.
Status:
ReviewerMember since: 2018
Organization: University of Barcelona, Bellvitge University Hospital, IDIBELL, CIBERNED
excellent results
Application Area:immunofluorescence and confocal microscopy
"Very useful as nuclear marker in well-preserved tissue sections and in cell cultures. Not suitable in tissue sections pre-treated with formic acid (as those assessed in human prion diseases to avoid "infectivity" of PrPSC)"
Manufacturer's ResponseThank you for your review and the valuable information on the incompatibility of DRAQ5 with formic acid-treated biohazard samples.
Status:
ReviewerMember since: 2018
Organization: Indian Institute of Technology Bombay
very useful and provides an opportunity to study hos-pathogen interaction in live.
Application Area:bacterial pathogenesis and host-pathogen interaction
"We used DRAQ5 for labeling our bacteria of interest for live imaging the bug during its intracellular life inside host cells. The excitation and emission spectra are sufficiently different compared other fluorescent compunds allowing it to be used in conjunction with other fluorescent dyes. We are currently exploring the possibility of employing DRAQ5 in fixed cell fluorescence imaging."
Manufacturer's ResponseThank you Anirban! Yes, DRAQ5 will also work for fixed cell fluorescence microscopy. You can use it prior to fixation, but more usually it is used AFTER fixation of the sample. We also have a new convenience alternative - DRAQfx FIX & GO - which has been specifically designed for the latter use and may exhibit less cytoplasmic background, though typically this is extremely low.
Status:
ReviewerMember since: 2018
Organization: University of Pennsylvania
Draq5 is a great live cell nuclear stain
Application Area:Airway cell nuclei stain
"Draq5 is an easy to use and affordable live cell stain."
Status:
ReviewerMember since: 2018
Organization: Université de Genève
I am really happy with this product.
Application Area:Flow cytometry immunology
"I am really happy with the use of this product allowing the elimination of dead cells for flow cytometry analysis."
Status:
ReviewerMember since: 2018
Organization: Newcastle University
Quick and easy to use stain.
Application Area:Immunofluorescence
"The product is very easy to use and gives accurate values for cell normalisation when compared to other nuclei stains. The use of draq5 in a plate reader really speeds up data acquisition."
Status:
ReviewerMember since: 2018
Organization: University of Minnesota
Excellent product for use in live-cell applications.
Application Area:Analyze protein subcellular compartmentalization in live cells
"We used this product to define nuclear localization of different proteins in cultured adult mouse cardiac myocytes. Draq5 was easy to use and provided a good fluorescent signal in the far-red spectrum that didn't interfere with our other fluorophores."
Status:
ReviewerMember since: 2018
Organization: University of Cambridge
Reliable and robust product.
Application Area:Host-pathogen interactions - immunofluorescence
"Great results enabling us to reproducibly track Chlamydia trachomatis during its interaction with the host cell. Reliable and robust product."
Status:
ReviewerMember since: 2018
Organization: Unidad de Investigacion Medica - Zactecas IMSS
Good results and works fine for live-cells. For fixed cells I rather use DRAQ7.
Application Area:Confocal microscopy
"DRAQ5 stain nuclei in an easy way, I have no doubt that is one of the best, mainly in live cells, though for the specific case of my lab we rather used cell-fixed preparations, though for this case DRAQ5 works fine as well. I'd recommend DRAQ7 which is a bit better. Cons, both of them are expensive in caparison with other nuclei staining dyes."
Status:
ReviewerMember since: 2018
Organization: EPFL
Very efficient and reliable reagent, easy to use and versatile
Application Area:Flow cytometry: discriminating nucleated cells from anucleate cells
"DRAQ5 from BioStatus is a very useful reagent for cytometry-related applications. For our study, it allowed us to discriminate between nucleated cells and anucleate cells. In addition, within the anucleate cells, it allowed us to discriminate between erythrocytes and reticulocytes. This reagent is very easy to use, extremely reliable and efficient as it quickly enters cells. It displays a very bright fluorescence with only a very small amount. Support from BioStatus is outstanding. It is also very easy to use and does not need to be washed."
Status:
ReviewerMember since: 2018
Organization: University of Padua
Good, but expensive. Recommended for live cells imaging
Application Area:Staining of cell nuclei for CLSM analysis
"The product works very well and can be re-used. I used it as an alternative to DAPI and Hoechst, to stain cellular DNA within the nucleus, because the CLSM system we use does not have a 405 nm laser line. The main problem of this product is that it is very expensive when compared to other stains such as the ones mentioned above. Indeed for such application I would not recommend it. However, it is ideal for live cell imaging experiments since it is cell permeable and the 633 laser used for its excitation is much less phototoxic as compared to the 405 one."
Manufacturer's ResponseDear Gualtiero, Thanks for your considered review of DRAQ5 and we note your comment on cost. DRAQ5 is second generation DNA probe that enables live or fixed cell counterstaining and avoids UV exposure as you rightly observe, plus with much lower photo-bleaching and remarkable shelf stability. Being red-excited it also enables use of the new violet-excited chromophores to extend channel capacity. Bearing these significant benefits in mind, the cost per slide of around 30 eurocents (based on 5uM, 200ul per slide, 200ul pack list price) would seem to be not unreasonable!
Status:
ReviewerMember since: 2018
Organization: Tel aviv university
Very good
Application Area:Nucleus staining for high throughput screening
"Reproducible staining and easy to use. I was very happy with the product"
Status:
ReviewerMember since: 2018
Organization: University of Guelph
Good results but kind of expensive
Application Area:Nuclear staining
"The stain is very effective and results in strong staining. We like it as it combines nicely with FITC and TRITC without bleed-through"
Status:
ReviewerMember since: 2018
Organization: Loyola University Chicago
Great results!
Application Area:Measuring nuclear expression of protein by amniss
"DRAQ5 was easy to use and produced high quality sharp staining for our analysis."
Status:
ReviewerMember since: 2018
Organization: Justus Liebig University
Great results with a simple and fast staining procedure.
Application Area:Stem Cell Research and Signal Transduction
"We have used DRAQ5 for several years in our laboratory and we are really satisfied with this product. It is very easy to use it with very fast staining procedure. It is not toxic at all for our cell model and also in the case of double or triple immunostaining, this DRAQ5 doesn't have any cross reactivity effect with other immunostaining. There isn't any after sales care for this special product, but it doesn't need it. It works perfectly without any complication."
Status:
ReviewerMember since: 2018
Organization: University of California, Irvine
Easy to use!
Application Area:Determine RBC counts in tissue samples
"This stain is very easy to use to stain for nucleated cells"
Status:
ReviewerMember since: 2018
Organization: Oniris
Easy to use and stable fluorescent signal
Application Area:Detection of draq5 labeled bacteria and staining of nuclei for cell and tissue phenotyping
"Draq5 is easy to use and give good repetability for nucleus staining in tissue section and fixed or living cells. This product has been used to label bacteria with success and was the best solution to detect bacteria on high autofluorescent wood. The far red emission of Draq5 is very interesting to use on autofluorescent tissue and his storage at 4°C allow to avoid too much cycles of freezing and thawing source of product alterations."
Status:
ReviewerMember since: 2018
Organization: LTRI
This product is reliable for efficient staining of the nucleus for live-cell imaging.
Application Area:Live cell imaging
"It was easy and effective to stain the nucleus. Staining protocol was simple to integrate into the work flow."
Status:
ReviewerMember since: 2018
Organization: University of Warsaw
Good value, great results, zero problems with the application.
Application Area:localization of cell nuclei
"We are using DRAQ5 for many years to label the cell nuclei in in vitro cultured cells and also in tissue sections. We did not experience any problems. Staining is consistent and easy to detect using confocal microscopy."
Status:
ReviewerMember since: 2018
Organization: Institute of Innate Immunity
I usually have really great images using Draq5
Application Area:Confocal microscopy
"Draq5 is extremely easy to use and gives immense signal with absolutely no background. It's a very powerful and useful dye that can be used to study cell nuclei morphology and identifying cell types."
Status:
ReviewerMember since: 2018
Organization: The University of Edinburgh
Works great! Results obtained with this product were used directly in a publication.
Application Area:Applied on thick live midgestation mouse embryo sections.
"After an initial titration for our specific tissue/cells, the DRAQ5 dye worked well and provided us with reproducible results. For our tissue (mouse embryo) we ended up using DRAQ5 at a 1:2000 dilution (1:500 proved to be too concentrated)."
Status:
ReviewerMember since: 2018
Organization: University G d'Annuzuio, Chieti
DRAQ5 is a very good tool to stain nucleus in live cells
Application Area:nucleus staining in live and fixed cells
"In PFA fixed cells DRAQ5 doesn’t gives always the same intensity; it works better in live cells. The staining in live cells is very fast and the nucleus appears very clear and bright."
Manufacturer's ResponseDear Silvia, thank you for the very constructive comments. We have developed DRAQFx as a standardized reagent specifically for the far-red nuclear counterstaining of fixed cells, as a ready-to-use dropper bottle formulation
Status:
ReviewerMember since: 2018
Organization: Indiana University
Easy to use and great results.
Application Area:Analysis of virus attachment on tissue culture cells.
"We use DRAQ5 to stain tissue culture cells for microscopy studies. The stain is easy to use and gives a great signal. The results are reproducible."
Status:
ReviewerMember since: 2018
Organization: University of Padova (Italy)
A really great product, excellent value for money
Application Area:Analysis of cell culture status.
"The product DRAQ5 is easy to use and gives excellent results in confocal microscopy. The protocols for multiple labelling are straightforward and can be applied to a variety of tissues/cells fixed in different way. In the lab, we used it on both tissues (normal or tumors) and cell cultures (cell lines and primary culture), always with success. A plus is the fact that the staining does not interfere with other staining, nor does it bleach other fluorophores."
Status:
ReviewerMember since: 2015
Organization: Imperial College London
Great results.
Application Area:immunofluorescence
"DRAQ5 is very user friendly. We used it at 2.5 microM concentration to visualize nuclear/mitotic DNA of U2OS cells. Incubation of DRAQ5 was done during the penultimate wash in our immunostaining protocol (incubation ~ 5min). Following a final wash in PBS, the slides were mounted and images acquired on an LSM510 confocal microscope."
Status:
ReviewerMember since: 2018
Organization: INSERM, France
Nice staining with no dirty background; very good results.
Application Area:DNA staining on retinal cell after transplantation
"very easy to use. very good reproducibility in different experiments. very cheap product."
Status:
ReviewerMember since: 2017
Organization: Institut für Immunolgie
great results
Application Area:Protein translocation using image stream
"The nucleus staining is very easy to use. It was stained 1:1000 in PBS Buffer and analysed by Amnis image stream"
Status:
ReviewerMember since: 2017
Organization: Feist-Weiller Cancer Center, LSU Health Sciences Center
Absolutely necessary to document movement of iron through intestinal cells
Application Area:Analysis of metabolites within cells
"Extremely easy to use. Highly reproducible results. Easy to quantify."
Status:
ReviewerMember since: 2017
Organization: Instituto Butantan
Excellent DNA dye - we use for single staining in antiparasitic high content assays
Application Area:Fluorescence microscopy in high content screening
"Ideal for single-staining high content assays, DRAQ5 enables clear visualization of cell nucleus and gives a smoother background signal at cell cytoplasm - which is enough for image processing softwares to delimitate and segment cellular structures properly. Moreover, the stain stability enables material storage for longer period than other conventional dyes (we have imaged 384-well plates after 4 weeks post-staining and the fluorescence signal was sufficiently high); also there is no need for dye washout after staining the microplates (less pipetting stesp). We recommend DRAQ5 in single-staning high content screening for assessing compound activity against intracellular trypanosomatids (Trypanosoma cruzi and Leishmania spp.) - the dye marks both DNA material from intracellular parasites and host cells - and providing a faint signal in the cytoplasm - turning it easy to segment and quantify the intracellular parasites."
Status:
ReviewerMember since: 2017
Organization: Tel Aviv University
Excellent cell imaging pictures
Application Area:Checking interactions between cells
"This dye helps us to check synapsis between mast cells and pancreatic cancer cells. These findings provide evidence for a novel mode of unidirectional crosstalk between MCs and cancer cells implicating direct activation by cancer cells in MC reprogramming into a pro tumorigenic profile"
Status:
ReviewerMember since: 2017
Organization: UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE
This is a product I intend to use, for as long as they produce it.
Application Area:HIV infection and signaling cascades in cell samples
"DRAQ5 is a simple, easy-to-use product. Either used on live-primary or fixed cells a simple 5-10 minute incubation followed by washing steps produced consistent, repetitive results. Its fluorescent excitation/emission spectra are very well suited to combine with other fluorophores without channel bleeding or crosstalk. The particular spectral characteristics of DRAQ5 make it also easy to work with in fluorospectrometry-based experiments. We have also used DRAQ5 combined with metabolic biomarkers in solution without observing interference with cellular metabolic uptake in short-time uptake experiments, this allowed us not only to reduce experimental time, but also helped us reduce cell manipulation."
Status:
ReviewerMember since: 2017
Organization: Middle East Technical university
Good results and quite easy to use
Application Area:Tissue Engineering
"For Draq5, it was easy to employ and visualize. As a fluorescent dye, the product was highly specific for DNA and there was no smearing problem"
Status:
ReviewerMember since: 2017
Organization: Anglia Ruskin University, Cambridge, UK
An excellent quality product that we will continue to use
Application Area:Imaging flow cytomtery for DNA repair biomarkers in human cells
"The Draq 5 product is of excellent quality and is reliable and reproducible under experimental conditions. In addition it is very easy to use. We have used the product for the majority of our imaging flow cytometry applications and have published extensively on this DNA repair/DNA damage and human genotoxicity. We will continue to use the Draq 5 formulation for our work although the price is rather too high"
Status:
ReviewerMember since: 2017
Organization: Tübingen University, Tübingen, Germany
Great results
Application Area:Confocal microscopy
"Excellent product with reproducible data. I would recommend to use other researcher"
Status:
ReviewerMember since: 2017
Organization: Androvia LifeSciences
Overall the product was effective.
Application Area:Analyze acrosome exocytosis in sperm
"Product was easy to store/handle. Purchase of product was easy. Preparation of the product took more time than expected but was due to the experimental protocol and not the product itself. We would purchase this product again for further research if needed."
Status:
ReviewerMember since: 2017
Organization: Goethe University - Frankfurt am Main (DE)
Easy to use reagent. Really precise and perfect for in-cell western experiments!
Application Area:Normalisation of cell number during in-cell western blot experiments
"I used DRAQ5 for internal normalisation of in-cell western (ICW) experiments to detect cytosine modifications in human cardiac mesenchymal cells. It arrived few days after my order. It is really easy and fast to use: it can be added at the end of the standard ICW procedure before plate reading. Really accurate and reproducible."
Status:
ReviewerMember since: 2017
Organization: University of Bristol
Nice reagent for cell cycle analysis
Application Area:Analysis of cell cycle status in colorectal cancer cells
"DRAQ5 is a useful reagent for analysing the cell cycle status of live/dead cells by flow cytometry. For our purposes, cells were first killed by fixation in 70% Ethanol (because we were co-staining for other antigens) and eventually resuspended in buffer containing 2ul/ml DRAQ5 allowing 10 mins prior to flow cytometric analysis. Lovely DNA profiles were obtained with no compensation issues with the other fluorochrome (PE) used in the study. Never attempted on live cells but we understand from other colleagues in the department that this is possible. Care must be taken when handling as this is a toxic substance."
Status:
ReviewerMember since: 2017
Organization: CFRC
Essential dye for my laboratory
Application Area:Live staining of nuclei for cell imaging
"DRAQ5 is consistently easy to use and produces excellent staining of live cell nuclei, for living cell imaging."
Status:
ReviewerMember since: 2017
Organization: Cancer Research Institute of Salamanca CSIC-USAL University
Easy to analyze apoptosis in cells at certain phases of the cell cycle
Application Area:Analyze apoptosis in cells at certain phases of the cell cycle
"The product is very easy to use for cytometry and results are reproducible. We perform simultaneous staining with Draq5 and Annexin-V on multiple myeloma cell line exposed to drugs and we follow their apoptosis at certain phases of the cell cycle."
Status:
ReviewerMember since: 2017
Organization: Heriot Watt University
Good nuclear stain, very easy to use.
Application Area:Nuclear staining, used together with polyclonal Ab and phalloidin, for confocal microscopy
"Effective and easy to use, the signal could be weak sometimes, but this could have been experimental variation or the microscope itself. Had no problem being able to pseudo-colour and the images and contrast with other stains was generally very good. The sales/technical team were very good before purchase, after-sales care not really applicable, as we didn't need to get in touch after buying it."
Status:
ReviewerMember since: 2017
Organization: Griffith University
Good nuclear stain that fluoresces in the far red.
Application Area:High content imaging.
"DRAQ5 is great as it can be used to stain living cells as well as fixed ones. Of course, the cells won't divide in it, so you can't use it for long-term time-lapse imaging, but it's still very useful for shorter time lapses."
Manufacturer's ResponseDear Bilal, thanks for your helpful feedback on DRAQ5. This is important information – we do not recommend DRAQ5 for long-term exposure to live cells as it, like ALL high affinity DNA binders and intercalators, will have some kind of impact on cell health. There are exceptions especially with terminally-differentiated cells as observed with HUVEC, macrophages and BaF3. However, short-term exposure and use in live-endpoint assays has been a hallmark of the product’s success in drug discovery and flow cytometry applications, in particular the loss of cytoplasmic GFP signals when cells are partially permeabilized by fixation with formaldehyde and the unspecific loss of cells due to RBC lysis and associated washes, respectively.
Status:
ReviewerMember since: 2017
Organization: University of Ottawa
Perfect for our needs, and gave consistent results.
Application Area:Nuclear staining of primary fibroblasts
"Product was straight forward to use, fast and consistent. Perfect for the application we required as a counter-stain for immunofluorescence using green and red fluorophores. Product lasted a long time (if stored correctly) and gave robust results until the last drop. The instructions which came with the product were straight forward and easy to follow for consistent results."
Status:
ReviewerMember since: 2017
Organization: Universitatea de Ştiinţe Agricole şi Medicină Vete
I loved the images obtained from the DRAQ5
Application Area:in vitro aplications
"We had very nice results using DRAQ 5 and we have published recently a paper. The price was a bit high."
Status:
ReviewerMember since: 2017
Organization: Erasmus MC, Immunology
Easy to use dye for analysis of DNA content without compensation issues in flow.
Application Area:immunology, B-cell development and leukemia
"Easy to use, good staining, no compensation issues in flow cytometry."
Status:
ReviewerMember since: 2017
Organization: University of Agricultural Sciences and Veterinary Medicine
Great product! It is absolutely vital for our lab, no nice photos without DRAQ5!
Application Area:Analyze cell culture metabolites - confocal microscopy
"The product is very easy to use for microscopy, in few minutes your sample is colored. I will recommend to researchers to use it if you have laser or filters for it. It is reliable and results are reproducible. We perform studies on melanoma and retina cell culture, exposed to different stress conditions and we follow their morphology after treatment."
Status:
ReviewerMember since: 2017
Organization: Wayne state university
great images and can use in far red region along with green fluorescent fluorophores
Application Area:To view the nuclei for imaging in confocal microscopy in the far-red region
"easy to use and high reproducibility and can image immediately."
Status:
ReviewerMember since: 2017
Organization: Bambino Gesù Children's Hospital and Research Institute
Great results
Application Area:Confocal Microscopy
"DRAQ5 is a good staining to see nucleus in liver cells and tissues. It is efficient in both paraffin-embedded and OCT-frozen tissue sections. It is easy to use and the results are excellent."
Status:
ReviewerMember since: 2017
Organization: Baker Heart and Diabetes Institute
An excellent product
Application Area:Visualize nuclei using confocal microscopy
"We used DRAQ5 to label the nuclei of live adult rat ventricular myocytes (see Weeks et. al. β-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC5) Nuclear Accumulation in Cardiomyocytes by B55α-PP2A-Mediated Dephosphorylation. Journal of the American Heart Association. 2017 6(4)). We labelled the cells with 1 uM DRAQ5 for 10 minutes prior to imaging, which was lower than the recommended concentration, but it fluoresced nicely."
Status:
ReviewerMember since: 2017
Organization: The University of Edinburgh
Great results
Application Area:To analyze nuclei in the cultured isolated bovine chondrocytes
"The dye was highly effective as even at very diluted concentrations produced excellent results. The confocal images obtained from these chondrocytes whose nuclei were labelled with DRAQ5 produced high quality and reproducible results. I had used this dye to count the number of cells in clusters because in my culture experiments the cell membranes were frequently touching and therefore thresholding of individual cells was impossible. In order to count number of cells in clusters I counted number of nuclei and DRAQ5 gave me highly reliable results as I could count the number of nuclei in 3D by using Volocity (a 3D image analysis software). It is quite an expensive dye but as the very diluted concentrations are very effective therefore, justifies the cost. Moreover, it was easy to use and highly penetrable and required very little time to label nuclei efficiently."
Status:
ReviewerMember since: 2017
Organization: UMCG
Nice alternative to DAPI.
Application Area:analyse transplanted tissue
"Fairly easy to use. Need to be made clear not to wash slides after DRAQ5 staining or colouring will be lost."
Manufacturer's ResponseDear Sarah, Thank you for your helpful reminder. We do indeed emphasise on our official technical datasheet that one should use DRAQ5 last and refrain from washing slides thereafter; if necessary any excess liquid can be tamped away. Kind regards, Roy Edward Sales & Marketing Director
Status:
ReviewerMember since: 2017
Organization: Institute of Biochemistry and Molecularbiology
Great and reliable results
Application Area:Nuclear staining in fixed cell
"The product is very easy to use. The results are very good and reliable in PFA and methanol fixed cell,. The fluorescence signal is of high qualitiy."
Status:
ReviewerMember since: 2017
Organization: KAIST
It gives us great results, thanks.
Application Area:Analyze reactive oxygen species in cells
"The product is easy to use and well-fit for your purpose."
Status:
ReviewerMember since: 2017
Organization: University of Miami School of Medicine
Great and dependable nuclear dye
Application Area:Immunofluoresence
"This product is very easy to use. Gives great reproducible nuclear staining. We have used this product both in immunofluorescence as a subcellular localisation marker and as a reference dye for plate based assays using a fluorescent reader (In-cell ELISA, ...)."
Status:
ReviewerMember since: 2017
Organization: University of Melbourne
Reliable and sensitive DNA stain
Application Area:immunofluoresence high throughput image analysis
"We found this product reliable and very easy to use. It worked consistently and we were very happy with its application. The product is quite expensive but we found we were able to use it at a lower concentration than recommended with just as great results."
Status:
ReviewerMember since: 2017
Organization: Institute of organic chemistry and biochemistry
A very useful product for live cell imaging.
Application Area:Microscopy
"The dye gives strong staining and could we washed away even from live cells. Nuclei of some cell lines e.g. PC3 could not be stained (the dye does not enter the cells) others e.g H1299 contain strange dotty staining in the cytosol in addition to the nuclear staining. Too high concentration causes artificial condensation of chromatin."
Status:
ReviewerMember since: 2017
Organization: University Health Network
Works well for cell proliferation assays
Application Area:Quantify cell numbers in response to small molecules in a high-throughput format
"This product allowed us to perform screens of compounds on cells that could not be assayed using traditional cell proliferation assays like Alamar Blue or MTT. The dye allows rapid quantification of cell numbers in a 96-well plate format. Assay of wells with multiple cell doses generated nice curves that gave us confidence in the quantitative ability of the assay over a wide dynamic range of cell numbers."
Status:
ReviewerMember since: 2017
Organization: NCI
Very useful! Using it a lot.
Application Area:DNA staining for high-throughput imaging
"Great staining and consistent results. Have used DRAQ5 for the past 5 years."
Status:
ReviewerMember since: 2017
Organization: Indiana University
Easy to use visualization of DNA
Application Area:stain DNA in living cells
"Easy to use, good brightness with limited photobleaching and phototoxicity"
Status:
ReviewerMember since: 2017
Organization: UConn Health
Great and reliable results. Highly recommended.
Application Area:To demonstrate binding of extracellular vesicles to leukocytes
"DRAQ-5 is easy to use and highly reproducible. it is a go-to reagent for nuclear identification."
Status:
ReviewerMember since: 2017
Organization: Institute for Stem Cell Biology and Regenerative Medicine
very useful chemical
Application Area:Analyze cell cycle and otehr parameters in cultured cells
"Excellent results with this consumable, we use it routinely"
Status:
ReviewerMember since: 2017
Organization: University of Camerino, MC, Italy (Now @ UTSouthwestern Medical Center, Dallas, Texas)
Great reagent, definitely worthy to be purchased and used!
Application Area:Confocal microscopy on Breast Tumor and Lung metastases sections
"DRAQ5 is a valuable alternative to DAPI if you want to use a red fluorescent dye for staining your nuclei. Its low-photobleaching, easy to use and yields a low background noise."
Status:
ReviewerMember since: 2017
Organization: INSERM
Good staining and good tools for IHF/IF
Application Area:Visualize nuclei in cells and tissus samples
"DRAQ5 is easy to use and fast (30min of incubation) and it stains well."
Status:
ReviewerMember since: 2017
Organization: University of Bern
Great results
Application Area:Analysing DNA in trypanosoma brucei
"It is a product, which is easy to use and gives a strong signal. We used it to stain the nucleus in T.brucei and it is compatible with the oregon green antibody for STED."
Status:
ReviewerMember since: 2017
Organization: Institute fo Research and Development
Must have tracer
Application Area:nuclear imaging
"Easy-to-use nuclear marker when other fluorescence emissions than blue (DAPI, Hoechst) are required."
Status:
ReviewerMember since: 2017
Organization: University of Miami
Easy to use, reliable, gives consistent results.
Application Area:To stain nuclei in fixed cell culture samples
"It is very easy to use, gives reproducible results, excellent product! Good value for the price too. Thank you!"
Status:
ReviewerMember since: 2017
Organization: RWTH Aachen University
Good reproducible results, more comfortable and suitable than DAPI staining
Application Area:Nuclear staining for indirect immunofluorescence experiments
"DRAQ5 was a very useful and easy to use tool for nuclear staining. In my immunofluorescence experiments I need to stain the nuclear fraction of the cells to have appropriate controls and DRAQ5 gave me this opportunity. Moreover, since the detection is at 633 nm laser range, I can use it instead of DAPI and use 405 nm channel for the detection of other dyes, such as Alexa Fluor 405"
Status:
ReviewerMember since: 2016
Organization: University of Barcelona
Very useful tool, easy to use and it guarantees good results!
Application Area:Analyze the cytotoxicity of compounds in live cells
"It is a very easy to use product. This provides high quality results and images working with live cells. "
Status:
ReviewerMember since: 2016
Organization: GPB Scientific
Great product.
Application Area:Identification of nucleated cells by flow cytometry
"Chose DRAQ5 because of its far red emission and near compensation free properties on the system I was using. The ability to get total nucleated for gating in log space and then DNA index with careful use in a slightly different red channel using linear is a nice plus."
Status:
ReviewerMember since: 2016
Organization: Hannover Medical School
Very good and reliable reagent.
Application Area:Immunocytochemistry, immunohistochemistry
"Easy to use and cost effective. Great alternative to traditional DAPI in immunocyto-and histochemistry."
Status:
ReviewerMember since: 2016
Organization: University of Toronto
Best nucleic acid stain
Application Area:Staining nuclei in 3D tissue constructs (tissue engineering application)
"Product (DRAQ5) nucleic acid stain works exceptionally well, and keeps common microscope filter channels open since it's a far red stain. Little to no background, easy to use and store. Penetrates deep into thick tissues so you don't observe staining concentration gradients as with other nuclear stains. Perfect for use when imaging 3D tissue constructs with confocal - but also works well for 2D monolayer applications where UV light isn't an option (ex: PMMA microfluidic devices which block UV)."
Status:
ReviewerMember since: 2016
Organization: MIT
Very useful for in vivo staining, easy to handle, high-quality
Application Area:Staining living mouse embryonic fibroblast nuclei
"If you are looking for a dye for in vivo staining, DRAQ5 is the best. Even with very little amount of a stock of the product, like 1ul, you can observe bright nuclei immediately. Staining genomic DNA specifically you could capture where DNAs are distributed in nuclei. After decorating your nuclei with it, cells are still happy living healthy. I will keep using the product for my future projects."
Status:
ReviewerMember since: 2016
Organization: Adam Mickiewicz University, NanoBioMedical Centre
Great results, easy to apply.
Application Area:Analyze toxicity, analyze the process of apoptosis
"High quality, easy to use, without non-specific staining, good efficiency."
Status:
ReviewerMember since: 2016
Organization: Wayne State University
DRAQ5 is the perfect nuclear stain if no UV-laser is available
Application Area:Nucleus staining in confocal microscopy
"I like that we can stain and visualize nuclei without the need for a UV-laser. The use is very simple, and the sensitivity is very good. We can also differentiate between DRAQ5 and far red probes so we can image several probes in parallel. I also like the fact that the company cares about publications with their products."
Status:
ReviewerMember since: 2016
Organization: UT Health Science Center at Houston
Good Product. Easy to use.
Application Area:Staining of Mouse ESC
"Its very easy to use, straight from the fridge without hassle and its reliable."
Status:
ReviewerMember since: 2016
Organization: Vanderbilt Uiversity
We achieved great and specific nuclear staining with this product
Application Area:Immunofluorescence Microscopy
"This product is very effective. It is also very potent and has high efficacy when used in amounts under the recommended concentration. Also, the fact that it can be used in live cells is a huge benefit."
Status:
ReviewerMember since: 2016
Organization: University of Pecs, Dept. of Pathology
Easy to use DNA stain for debris discrimination.
Application Area:Flow cytometry
"We use DRAQ5 for debris discrimination in CD34+ cell enumeration tests of blood and harvest samples using lyse no wash method. It works very well combined with CD34 PE and CD45 FITC."
Status:
ReviewerMember since: 2016
Organization: University of New Brunswick
Great results
Application Area:Cell culturing for evaluation of biocompatibility
"Easy to use, fast shipping, reasonable price, good results and good quality"
Status:
ReviewerMember since: 2016
Organization: Wayne State University
Overall very satisfied with the product and will recommend to all users.
Application Area:Confocal Microscopy
"The product works great for our applications such as confocal microscopy. We rate this product the best among all the manufacturers. Overall we will order and use this product as long as the prices are kept competitive or we have more superior advantage."
Status:
ReviewerMember since: 2016
Organization: UT MD Anderson Cancer Center
Good product.
Application Area:Analyze CTC from blood.
"Easy to stain. It was used for stain the nucleus of cells for confocal microscopy."
Status:
ReviewerMember since: 2016
Organization: Helen F. Graham Cancer Center & Research Institute
Great nuclear dye!
Application Area:Immunofluorescence
"Results are quite reproducible and DRAQ5 is easy to use."
Status:
ReviewerMember since: 2016
Organization: Wake Forest University Health Sciences
This product performed reliably and consistently!
Application Area:Positive control antibody
"We used DRAQ5 as a nuclear stain to normalize for well-to-well variations in cell density. This was done in an On-Cell western looking at expression of CB1 surface receptor. This product was consistent in staining and was an excellent reference stain."
Status:
ReviewerMember since: 2016
Organization: Oxford Brookes University
Great results, highly recommend it.
Application Area:Staining of nuclei in live plant cells for visualization of mitosis
"I used this product to stain nuclei in living cultured plant cells for confocal imaging of mitotic events. I found the dye easy to use and the staining was very quick. The fluorescence was strong and very stable."
Status:
ReviewerMember since: 2016
Organization: Harvard Medical School
Great dye for live or fixed cell imaging.
Application Area:Cell segmentation
"Great dye for live or fixed cell imaging. Especially useful for nuclear and whole cell segmentation."
Status:
ReviewerMember since: 2016
Organization: Jawaharlal Nehru University, New Delhi, India
Great results. Would recommend to the entire community
Application Area:Confocal imaging of parasites
"Excellent product. I had earlier used Giemsa for staining the parasite in macrophages. I work on a protozoan parasite and once I worked with DRAQ5 phew I got my paper accepted in Autophagy. Would recommend to all."
Status:
ReviewerMember since: 2016
Organization: university of houston
DRAQ5 and Retina
Application Area:Immunohistochemistry
"This is an excellent easy to use marker of cell bodies, especially in retina and brain"
Status:
ReviewerMember since: 2016
Organization: MTA-TKI
Nice images with confocal laser scanning microscopy
Application Area:Tumor cell lines
"Worked with diluted stock solution in PBS (better handling and accuracy). The applied incubation time, temperature and concentration were important for good reproducibility. On permeabilized cells the nucleus had better signal and stained clearly. On the non-permeabilized cells, the cytosol was stained also."
Status:
ReviewerMember since: 2014
Organization: Universiti Putra Malaysia
Great data achieved, images clear
Application Area:Trafficking in cells
"Very effective , very ease of use and one bottle can provide thousands data..."
Status:
ReviewerMember since: 2016
Organization: University of Zaragoza
Great results and easy use.
Application Area:See different nuclei cells types
"The react is easy to use and very effective. We can use it for different cells types and in different conditions."
Status:
ReviewerMember since: 2016
Organization: Jacobs University
Recommended
Application Area:Staining Nucleic Acids
"We used DRAQ5 for staining of nuclear DNA - and realized that it also nicely stains RNA in Dictyostelium cells, which, for our purpose, was beneficial."
Status:
ReviewerMember since: 2016
Organization: Center for Cancer Research
DRAQ5 is vital to analyze each sample I process
Application Area:Flow cytometry
"I use DRAQ5 in order to separate living cells of dead cells in a suspension of brain tumor cells and to calculate the cellular DNA content. I use 3 ul of DRAQ5 3 minutes before the acquisition, therefore I spend several vials of DRAQ5 in a short time and it is an expensive dye."
Status:
ReviewerMember since: 2016
Organization: UMR703 PAnTher
Great result, applicable in all of our research
Application Area:Analyse dystrophin in tissue sample
"High quality, reproducible, easy to use, high intensity"
Status:
ReviewerMember since: 2016
Organization: MGH
Good nuclear stain
Application Area:Separate two populations based on DRAQ5+/-
"Good alternative to Hoechst, DAPI for multiplexing. Stained cells must be washed multiple times to avoid dye leakage into unstained cells."
Status:
ReviewerMember since: 2016
Organization: Vanderbilt University
Good results, bio-safe, easy to use, reliable, helped in visualization of nuclei in live HeLa cells and calculation of Nuclear vs Cytoplasmic ratio of LC3 using MATLAB.
Application Area:Nuclei visualization in live HeLa cells
"DRAQ5 was used to visualize nuclei in live HeLa cells using confocal fluorescence microscope LSM 510. This product is very stable and convenient to use. The protocol involving dilution and staining is very simple and easy to follow. The dye is not toxic to HeLa cells and bio-safe. The dye did not interfered with subsequent downstream biochemical analysis e.g. western blot and protein band detection by LI-COR. DRAQ5 uses far red wavelength, it does not involve the risks associated with UV light as associated with some other nuclear staining dyes in market. Also it was advantageous to us since nuclei visualization was the most essential part of our experiments and DRAQ5 imaging was compatible with our LSM 510."
Status:
ReviewerMember since: 2016
Organization: IHiT
Great product, worth the price.
Application Area:Immunofluorescence - nuclei staining
"DRAQ5 is easy to use, gives reproducible results. Excellent product - good replacement of DAPI."
Status:
ReviewerMember since: 2016
Organization: University of Pittsburgh
Bright signal. More consistent and lower spillover than DAPI.
Application Area:Nuclear marker in localization studies (ImageStream), Tissue ImmunoFluorescence
"We used DRAQ5 as nuclear staining in Flow Cytometry and ImageStream analysis to track the subcellular localization of different proteins. It works well with different detectors and offers a nice alternative to DAPI, leaving UV fluorophores available for other markers. This product is also very convenient in tissue immunofluorescence as a cellular marker (nuclei). It allows easy characterization of immune infiltrates in diverse tissues with better and more consistent signal than DAPI."
Status:
ReviewerMember since: 2016
Organization: Medical Universtiy of South Carolina
Perfect for nuclear staining!
Application Area:Molecular mechanisms of cell signaling and nuclear/cytoplasmic localization
"I have been using this product for past 6 years and it is reliable, perfect and very easy to use."
Status:
ReviewerMember since: 2016
Organization: MTA TTK, Hungarian Academy of Sciences
Great and reproducible results, I recommend the use of DRAQ5.
Application Area:Staining of DNA in rat leukocytes
"We used DRAQ5 staining for the validation of FSC/SSC dot plot based identification of rat leukocytes from lysed whole blood sample. The use of DRAQ5 is easy, it stained the nuclei instantly. We used DRAQ5 several times and achieved always similar staining intensities, indicating good reproducibility."
Status:
ReviewerMember since: 2016
Organization: University of Washington
Very happy with this product.
Application Area:Microscopy.
"Very easy to use. Consistent nuclear staining for fluorescent microscopic analysis of cells and FFPE tissue."
Status:
ReviewerMember since: 2016
Organization: Institut National de la Recherche Scientifique
Excellent product that gives highly reproducible results.
Application Area:Confocal microscopy looking at viral replication in neuronal cells in culture.
"We used DRAQ5™ to stain the nuclei of neurons maintained in culture that were infected with herpes simplex virus 1 and processed for indirect immunofluorescence. Samples were analyzed by confocal microscopy."
Status:
ReviewerMember since: 2016
Organization: University of Missouri-Columbia
Good product.
Application Area:Nuclei staining for microscopy imaging analysis.
"It is easy to use. It can substitute DAPI, but it is the far-red that occupy one channel for other fluorophores. I used it for certain microscope that did not have DAPI channel."
Status:
ReviewerMember since: 2016
Organization: NanoBioMedical Centre, UAM
Great results and no time consuming staining.
Application Area:Analyze morphology of human cells/ analyze the influence of nanomaterials on cells.
"We noticed a good staining with DRAQ5™ of cell nuclei and repeatability of experiment. We obtained a good signal under the confocal microscopy. The DRAQ5™ dye doesn't bind unspecifically."
Status:
ReviewerMember since: 2016
Organization: Institute of Medical Biochemistry, ZMBE, University Muenster, Germany
Worked very nicely, we use it continuously.
Application Area:Analysis of cell division.
"The product is very easy to use and very efficient in its application. We have used it in the study of mitotic spindle orientation to identify the specific stages of mitosis. The product always worked very reliably in our hands. It could, however, a bit cheaper."
Status:
ReviewerMember since: 2016
Organization: Harvard University
Great label for the nucleus
Application Area:Marker for the nucleus
"DRAQ5 is a high quality far red nuclear label that we frequently use in semi-automated confocal microscopy experiments. The dye appears to behave very well--it is easy-to-use, stable, and provides a high quality label for the nucleus of live cells."
Status:
ReviewerMember since: 2016
Organization: Molecular Medicine Department - University of Padova
Great results
Application Area:Analyze nuclei in cellular preparations
"Very easy to use, have the great advantage to be usable in live cells."
Status:
ReviewerMember since: 2016
Organization: Brunel University London
Highly recommend it. Works great for ImageStream
Application Area:Stain ovarian cancer cells
"DRAQ5 is extremely easy to use for our application: i.e. staining nuclei of cells for high imaging flow cytometry. Reproducible results every time. "
Status:
ReviewerMember since: 2016
Organization: University Hospital Freiburg
we usually use it in routine analysis
Application Area:staining of living cells
"Very easy product for the staining of cells in FASC analysis."
Status:
ReviewerMember since: 2016
Organization: Tufts University
Solid results, quite pleased with this product.
Application Area:Analyze pore formation in host cells
"After assay optimization, this product worked quite well for imaging host cells by microscopy during bacterial infection. The product has a long shelf life, provided consistent results, and was not that expensive. The only reason I gave 4 starts for the After Sales Care is because I had no direct interaction with them, but I think our lab tech found the initial instructions with the package confusing. Overall, it is a great product for a specific assay."
Status:
ReviewerMember since: 2016
Organization: University of Heidelberg, Germany
Excellent product, would thoroughly recommend it
Application Area:Fluorescence labelling of cell nuclei
"Draq5 is reliable and easy to use. It has many advantages over other staining methods. We have used it for years now, and have always been happy with the results. It is now the standard staining methods for many techniques in my lab."
Status:
ReviewerMember since: 2016
Organization: 1989
Perfect Product
Application Area:Flow cytometry - To stain the nucleus of myoblast and myotubes cultures
"DRAQ5 is easy to use and it gives us a good reproducibility and effectiveness. In my experiments, it was a really helpful product to stain the nucleus of my cells in an easy way. In addition, the quality of DRAQ5 is sufficient high for a broad range of several flow cytometry experiments. I am very pleased with the product and would recommend it to anyone."
Status:
ReviewerMember since: 2016
Organization: Atilim University
The marker works very well for imaging of cell spreading and growth within and or on the scaffolds
Application Area:Cell adhesion and growth marker
"It is very easy to use and we obtained very significant results with the product"
Status:
ReviewerMember since: 2015
Organization: University of Munich
Works with 2-photon excitation at 1275 nm.
Application Area:DNA staining in 2P microscopy
"We used it for live DNA-staining in 2Photon microscopy with long wavelengths (1275 nm), so we could use it in parallel with Third Harmonic Generation (THG) detection. Worked as expected. "
Status:
ReviewerMember since: 2016
Organization: University of South Alabama
Outstanding results, isn't the cheapest nuclear label, but works well
Application Area:Live cell imaging
"DRAQ5 works well as a nuclear label and allows us to use a different spectral range for configuring our multi-label assays. It appears (not 100% sure why) that DRAQ5 has less particulate and non-specific labelling in our microvascular endothelial cells than Hoechst. "
Status:
ReviewerMember since: 2016
Organization: INSERM , Intitut de Recherche en Santé Digestive (IRSD), Toulouse, France
Draq5 is an easy and efficient fluorescent Live-Cell Permeant DNA Dye to perform accurate normalization in Cell Western Blotting.
Application Area:Cell number normalization of fluorescence signal between wells in cell Western Blotting
"The product Draq5 is a ready-to-use aqueous formulation really easy to use (simple incubation and washes) and it gives us a good reproducibility between experiment. It has a good stability after storage. Draq5 is a stable fluorescent Live-Cell Permeant DNA Dye which allow us to normalize the fluorescence signal between wells in an in Cell Western Blotting (Odyssey). We followed protein expression (Fluorescence 1) in bone marrow derived macrophages after different cell culture conditions and Draq5 signals (Fluorescence 2) provided us a good linearity for normalization of protein signals well-to-well (ration Florescence1/Fluorescence2). "
Status:
ReviewerMember since: 2016
Organization: Clinical Pharmacology and Toxicology, Hamburg
Nice option to visualize nuclei in living cells
Application Area:staining of nuclei in living cells
"We achieved reproducible results in a high quality. DRAQ5 was really easy to use. We needed small amounts (1:1000) to make nuclei visible during immunofluorescence analysis. An Incubation for 10 minutes with 1:1000 DRAQ5 in Medium was enough to stain nuclei and it wasn’t indispensable to change/discard medium for microscopy."
Status:
ReviewerMember since: 2015
Organization: University of Padua
Good results
Application Area:Analyze antigen in adrenocortical tissues
"Easy to use, with high quality of results. By Immunofluorescence analysis we have rapidly analyze the nucleuses of our cells. "
Manufacturer's Response"Thanks for taking the time to review DRAQ5. We would love to know whatwould make DRAQ5score 5’s–that would help direct our product development."
Status:
ReviewerMember since: 2015
Organization: Institute of Pathobiochemistry, Johannes-Gutenberg University Mainz
Very good results with Draq5
Application Area:analyzes of protein endocytosis
"Draq5 is easy to use and the staining of the nucleus is bright and specific."
Manufacturer's Response"Thank you for the positive response on the performance of the product! I’m sorry that we didn’t meet your after-sales expectations but have no record ofreceiving an after-sales enquiry. We would love to know what we could do better."
Status:
ReviewerMember since: 2016
Organization: Houston Methodist Research Institute
Good product for staining
Application Area:Nuclear staining
"The lab personnel have been requesting this product for the last few years for their research studies."
Status:
ReviewerMember since: 2016
Organization: NIH
Results were great, dye of choice for staining nuclei
Application Area:Staining nuclei in cardiomyocytes (engineered heart tissue)
"The value for the money is great - with only a tiny amount of dye the nuclei in our cardiomyocytes in the engineered heart tissue samples were stained in a high quality. We used it for an immunofluorescence staining of the whole-mount tissue, which worked out fine. We can highly recommend it."
Status:
ReviewerMember since: 2016
Organization: University of Tokyo
Great results
Application Area:Nuclear Stains
"We wanted to use far-red nuclear dye, because of the limitation of our old fluorescent microscope. DRAQ5 did quite well!"
Status:
ReviewerMember since: 2016
Organization: University of Colorado
Consistent staining of nuclei
Application Area:Immunofluorescence - Nuclear counterstain
"Extremely bright nuclear counter-stain, allows rapid and efficient nuclear staining for immunofluorescent applications. Extremely easy to use."
Status:
ReviewerMember since: 2016
Organization: Inserm U1118
Nice results in confocal imaging
Application Area:nuclear staining in histology
"Easy to use stain for nuclei. We used it to counterstain double IFs in confocal imaging. It worked beautifully."
Status:
ReviewerMember since: 2015
Organization: UW
The best way to visualize nuclei
Application Area:visualisation of nuclei during immunofluorescence analysis
"DRAQ-5 is really easy to use for visualization of nuclei during immunofluorescence analysis. For simple samples (cells in monolayers) it is great. However, for samples with many layers it does not penetrate all layers equally. For that reason, such "difficult" samples are not reproductive in 100%. "
Status:
ReviewerMember since: 2015
Organization: The Ohio State University
Great product!
Application Area:Analzyed ciliary muscle cell numbers
"The was an easy product to use. I needed to try a couple of different concentrations; however, after I found the correct concentration, I was easily able to visualize ciliary muscle cell nuclei. "
Status:
ReviewerMember since: 2015
Organization: Institute of Endocrinology and Molecular Oncology. National Council of Research. Napoli-It
It is a great reagent. Indispensable if you haven't a UV laser.
Application Area:Nuclear staining in immunofluorescence
"DRAQ5 is very easy to use. It gives always reproducible results. we never needed after sales care. It is quite expensive. "
Status:
ReviewerMember since: 2015
Organization: Tel Aviv University
excellent product
Application Area:Immunoflourescence of tissue cultured cells
"A good product. Gives excellent results in no effort. Very useful "
Status:
ReviewerMember since: 2015
Organization: University of Warsaw
Very good results
Application Area:immunocytochemistry, confocal microscopy
"Allows us to achive reproduciple results and it is easy to use"
Status:
ReviewerMember since: 2015
Organization: RWTH Medical School, Institute of Biomedical Engineering, Dept. Cell Biology
excellent product
Application Area:microscopy, histology
"I rate DRAQ5 as an excellent product regarding easy to use. The price is fair and the after-sale support excellent."
Status:
ReviewerMember since: 2015
Organization: University of Colorado School of Medicine
We use this nuclear counterstain almost exclusively at this point
Application Area:frozen sections mouse tissue
"DRAQ5 gives us reliable nuclear staining in the long red which we can readily image with conventional fluorescence and a digital camera, and confocal microscopy."
Status:
ReviewerMember since: 2015
Organization: University of Nottingham
Application Area:Confocal Microscopy
"It is very easy to use, and I got very clear with high quality pictures by confocal microscope after staining cancer cells by DRAQ5."
Status:
ReviewerMember since: 2015
Organization: Instituto Ferreyra- INIMEC-CONICET-UNC
"We easily got good results. The product was easy to use, although for us in countries with devalued currency it is quite expensive. This is however the same for any imported reagent, so I would say the value for money was OK. "
Manufacturer's Response"Thank you for your review. BioStatus shares your concern regarding importation using weak currencies. It may be more cost-effective to purchase direct in GBP from BioStatus in these circumstances."
Status:
ReviewerMember since: 2014
Organization: MRC NIMR
Application Area:Fixed granulocyte DNA staining
"Good signal with no detectable bleed through in other fluorescence channels. Quick staining protocol, superior to other DNA dyes we’ve tested."
Status:
ReviewerMember since: 2015
Organization: "La Sapienza" University of Rome
Application Area:Staining of cell nucleus for immunofluorescence.
"DRAQ5 staining works very well in our experimental conditions even though with better results in non-neuronal cells. I also used DRAQ5 in concomitance with another nuclear marker with very nice outcomes. I would recommend it to other people for its easy handling and competitive costs. Thank You"
Status:
ReviewerMember since: 2015
Organization: Morehouse school of medicine
Application Area:immunology assays
"We have used DRAQ5 frequently for dendritic cells and T cells and epithelial cells nuclear stain. DRAQ5 is an excellent nuclear stain and easy to use compared to DAPI. The price is high but high dilution. So it is worth. "
Status:
ReviewerMember since: 2014
Organization: Norwegian University of Science and Technology
Application Area:Molecular Medicine
"DRAQ5 is easy to use. In my research, DRAQ5 was used to determine cellular DNA sets immediately after fluorescence measurement of cellular photosensitizer, giving quantitive analysis of drug uptake. The quality of experiment was excellent."
Status:
ReviewerMember since: 2015
Organization: Karolinska Institutet, MTC, Stockholm, Sweden
Application Area:Stain nuclei in immunofluorescence
"Very good nuclear stain in the far-red; the product of choice if you cannot use DAPI-related dyes."
Status:
ReviewerMember since: 2015
Organization: Swedish University of Agricultural Sciences
Very easy to use and stable.
Application Area:Staining of bovine blastocyst (confocal microscopy)
"Very easy to use (one step direct staining), did not work well with cat blastocyst though - we have no explanation why. Very stable (at least 6 weeks in refrigerator after mounting on slides). It took some time to order to Sweden (through middle steps, not sure if we used the right channels). Equivalent to Hoechst or DAPI but more expensive. Excellent for our confocal that does not have a blue laser."
Manufacturer's Response"Thank you for the feedback. We are sorry for the difficulty experienced in purchasing our product. We have a number of reputable, approved distributors listed on our website and failing that we supply direct, next day, around the globe on a daily basis."
Status:
ReviewerMember since: 2015
Organization: National Cancer Institute- Aviano
Application Area:Analyze cell viability and nuclei morphology
"Easy to use and sensitive. It’s a good dye to complete flow cytometry, confocal microscopy or multispectral flow cytometry evaluations. "
Status:
ReviewerMember since: 2015
Organization: University G d'Annunzio Chieti-Pescara
"We use DRAQ5 from many years both in human and mouse samples formalin fixed or in OCT with good results. I would recommend this product. It is not expensive and the results are good."
Status:
ReviewerMember since: 2015
Organization: University of Waterloo
We prefer to use DRAQ5 as a far-red nuclear stain for most of our confocal microscopy experiments involving gene transfection studies with a variety of nanoparticles.
Application Area:We use DRAQ5 as a nuclear stain for imaging in a variety of cellular drug delivery experiments.
"We actually prefer to use DRAQ5 as a far-red nuclear stain for most of our confocal microscopy experiments involving gene transfection studies with a variety of nanoparticles including lipid-based, peptide-based NPs and carbon nanotubes. We obtained excellent results in a variety of cell types in live cell assays and also in cryostat sectioned skin and eye tissues. We highly appreciate the prompt shipment and outstanding scientific support."
Status:
ReviewerMember since: 2015
Organization: Jacobs University Bremen
Application Area:Co-stainings for various mammalian cell lines and tissues (cryo- and paraffin sections)
"DRAQ5 is easy to use and gives highly reproducible results in various mammalian cell lines as well as tissue sections."
Status:
ReviewerMember since: 2015
Organization: Medical University of Innsbruck
Application Area:microscopy imaging
"We used DRAQ5 for nuclear counterstaining in filamentous fungi for imaging. DRAQ5 works perfectly using multiple fluorescent markers, since its far red emission avoids signals in other frequently used channels and gives bright signals. It is easy to use and ensures a long lasting fluorescence."
Status:
ReviewerMember since: 2015
Organization: EMD Milliopre
Application Area:To stain DNA in fixed human peripheral blood lymphocytes
"DRAQ 5 is an excellent product that is easy to use. I would recommend it to anyone wishing to stain DNA in live or fixed cells. One major benefit over other nuclear stains is that excess dye does not need to be washed out as is the case with many other DNA stains. We did not have any discussion with the company after purchase simply because none was necessary. "
Status:
ReviewerMember since: 2015
Organization: University of Colorado Anschutz Medical Campus
Application Area:Immunhistochemistry on frozen sections
"Easy to use (diluted in phosphate buffer; dilution factor can be increased if increasing incubation time ), sharp staining and contrast, extremely stable compared to other long-red nuclear counterstains we have tried in the lab for immunohistology (no need of special anti-fade mounting medium)."
Status:
ReviewerMember since: 2015
Organization: Karolinska Institutet
Application Area:for in cell western and total protein normalization
"Quite an easy to use product that you could use for total protein cell normalization during in cell western analysis."
Status:
ReviewerMember since: 2015
Organization: Instituto Cajal, CSIC
Application Area:Slices of fixed tissue
"DRAQ5 is a great alternative to label cell nucleus when you have other different fluorescent markers in the same sample. Its far red emission makes it compatible to other fluorophores of the spectra without crossing signals."
Status:
ReviewerMember since: 2015
Organization: Quintiles
Application Area:Fixed-cell staining of adherent cells
"DRAQ5 is easy-to-use and stains cells brightly. Great alternative when using multiple markers or if a stain has a large spectrum."
Status:
ReviewerMember since: 2015
Organization: Evotec
Application Area:High Content imaging, cell based assays
"DRAQ5 is our most established nuclear stain for cell based assays. It also stains the cytoplasm to some degree which is useful for cytoplasm detection. It's emission in the far red is useful as it does not cause cross signals on lower wavelength-excited channels (while Dapi or Hoechst do)! It is quite expensive though! After sales care not required. "
Status:
ReviewerMember since: 2015
Organization: Duquesne University
Application Area:For In-Cell Westerns on the Odyssey Imager
"Using DRAQ5 on the Odyssey to approximate cell numbers is not as sensitive as manual counts of nuclei on a microscope but is much faster and less biased."
Status:
ReviewerMember since: 2006
Organization: Indiana University
Application Area:for nucleus staining
"I use this often when I use microscope without filter for 350nm wave length. It is easy to use and stains very well. I always recommend this whenever there are people who want to use nucleus staining."
Status:
ReviewerMember since: 2015
Organization: Imperial College London
Application Area:Image stream, confocal microscopy
"We mainly use DRAQ5 as a nuclear stain for Image stream (cells in suspension). It is easy to use, and very bright."
Status:
ReviewerMember since: 2015
Organization: Institute of Human Genetics Heidelberg
Application Area:Hela cell staining
"Very easy to handle and worked every time we used it. Showed consistent and uniform staining intensity."
Status:
ReviewerMember since: 2015
Organization: University of Alberta
Application Area:Use to stain nuclei for imaging flow cytometry
"DRAQ5 is incredibly easy to use. Just dilute and add to cells immediately before acquiring days. Dye incorporates quickly into both live and fixed cells. It also gives a nice bright stain of the nucleus that has crisp edges. Post-sales, the company was always readily available for any questions or to help with designing experiments with any of their other products. DRAQ5 is excitement stable, even with left diluted at room temperature and very little dye is needed to stain cells."
Status:
ReviewerMember since: 2014
Organization: University of Michigan
Application Area:immunostaining
"Quick and very easy to use, little to no background. Tight wavelength makes for easy co-staining with other fluorophores"
Status:
ReviewerMember since: 2014
Organization: Université de Poitiers
Application Area:We used DRAQ5 in immunofluorescence studies.
"DRAQ5 enable us to efficiently stain the nuclei of our mammalian cells (prostate cancer and neuronal cell lines). We used it in a mix with our secondary antibodies."
Status:
ReviewerMember since: 2014
Organization: Wake Forest Health Sciences
Application Area:We use DRAQ5 in On- and In-cell-Western assays to account for wll to well variations in cell numbers
"Overall DRAQ5 is an excellent product with simple and easy to use instructions. The dye has very good sensitivity and specificity and can be easily plugged into any protocol with minimal change. DRAQ5 has cut down on protocol time and the high concentration of the stock solution allows one to conduct multiple studies with one vial of solution; thus dramatically reducing costs and time compared to similar nuclear dyes. "
Status:
ReviewerMember since: 2015
Organization: University College London
Application Area:Light microscopy and flow cytometry
"We need to counterstain for DNA in most of our experiments using human cells. DRAQ5 is rapid, simple and very efficient and, very importantly, requires a far-red excitation, much more common and less toxic for live cells than UV lasers (required for DAPI/Hoechst staining). "
Status:
ReviewerMember since: 2015
Organization: University Hospital Bonn
Application Area:Live cell nuclear detection
"We use DRAQ5 for high content screening in live HEK293 cells for the purpose of nuclear detection and segmentation. The dye very simple to use and works perfectly at very low concentrations in HEK293 cells with very low toxicity."
Status:
ReviewerMember since: 2014
Organization: Cincinnati Children's Hospital Medical Center
Application Area:We use DRAQ5 on the Amnis Imagestream to visualize nuclear translocation of transcription
"We have used DRAQ5 for Amnis ImageStream analysis for several years now and it has a nice consistently bright staining. We found this dye to be more reliable and less problematic (bleeding into subsequent samples, contaminating tubes, compensation issues) than various other dyes such as Dapi and hoechst dyes. We add 2-3ul of a 1:1000 dilution to our cells (1-2x10^6)/100ul just before acquisition. Officially our vial has expired (for at least a year), but we have seen no reduction in signal. We have used DRAQ5 for traditional flow-cytometry as well, to either eliminate or select cell-doublets or 2-cell conjugates. For flow we use a 10-fold higher concentration than for imaging, which makes it less cost-effective than some other live dyes in this setting."
Status:
ReviewerMember since: 2014
Organization: Louisiana State University
Application Area:Used as a nucleus stain
"Very effective in imaging, minimum interference with our emission wavelength we study. Easy to use, just minutes of incubation, no unnecessary waiting. I would highly recommend this product to fellow researchers in similar fields."
Status:
ReviewerMember since: 2014
Organization: UConn Health Center
Application Area:IHC of fixed mouse spinal cord tissue sections
"This dye nicely stains the nuclei even in 60 micron thick spinal cord tissue sections in just 10 minutes. It helped us quickly visualize the aggregation of leukocyte infiltrates across the spinal cord vessels in a mouse model of Multiple Sclerosis."
Status:
ReviewerMember since: 2014
Organization: Uconn health
Application Area:We are using DRAQ5 to look for infiltrating cells into the CNS parenchyma during neuroinflammation.
"This product is easy to use, very efficient and effective. It always has good staining results. "
Status:
ReviewerMember since: 2014
Organization: Karolinska Institutet
Application Area:Cell normalization in "in cell western"
"DRAQ5 worked really well as normalization in odyssey in cell western protocol."
Status:
ReviewerMember since: 2014
Organization: University of Otago
Application Area:In-Cell Western
"Our lab routinely uses DRAQ5 for cells normalisation using the In-Cell Western Technique. Overall, we are satisfied, the product works well, it is easy to use and it works very well with the Odyssey Classic - Detection System based on Infrared Technology."
Status:
ReviewerMember since: 2014
Organization: FONDAZIONE IRCCS ISTITUTO NAZIONALE DEI TUMORI
Application Area:CELLULAR BIOLOGY
"DRAQ5 is a trouble-free and versatile reagent to be used in immunofluorescence analysis. It is a high quality product. "
Status:
ReviewerMember since: 2014
Organization: National University of Singapore, Department of Chemical & Biomolecular Engineering, 4 Engineering D
Application Area:biomedicine
"This is easy to use with high imaging resolution and is good value for money."
Status:
ReviewerMember since: 2014
Organization: Eötvös Loránd University, Budapest, Hungary
Application Area:cell biology, immunology, receptor-signaling research
"This product is very useful for us in intracellular staining of receptors for localization. It is an easy to use nuclear counter stain because of its advantageous spectral properties (it does not cover at least two or three out of the available visible range optical channels in confocal microscopy, like propidium iodide). Sometimes we can also utilize its unique property that it does not need cell permeabilization that other nucleic acid stains require."
Status:
ReviewerMember since: 2014
Organization: Universiti Putra Malaysia
Application Area:Bio-nanotechnology
"It is very easy to use, and very versatile. It gives good signal without photo-bleaching. I used Draq5 for both fixed and live cell imaging. I recommended to other researchers who is involves in live cell imaging and those working on drug delivery."
Status:
ReviewerMember since: 2014
Organization: Åbo Akademi University Turku Finland
Application Area:Immunohistochemistry
"I have been doing immunohistochemistry for many years and found that DRAQ5 nuclear stain is far better than other nuclear stains as it provides better resolution and staining results."
DRAQ5™ is a novel far-red fluorescent LIVE cell DNA dye that can be used in combination with other common fluors, especially GFP fusions & FITC-tags.
DRAQ5™ has many applications and is highly compatible with existing protocols across a wide range of instrumentation platforms. Biostatus is happy to provide extensive pre-sales technical support for your application.
Main application areas are HCS, flow cytometry and fluorescence microscopy.
FOR HCS LABS:
DRAQ5™ is the ideal nuclear counterstain for HCS.
It can reduce the screening time by 50%.
It permits simultaneous visualisation of the cytoplasm and the nucleus with one fluorophore!!!
Suitable for all HCS platforms including GE InCell 3000 and Evotec Technologies' Opera.