Deoxyribonuclease I (Dnase I) by Worthington Biochemical Corp.

Product Codes: DPRF and DPRFS are both especially designed for Molecular Biology applications and contain the lowest levels of ribonuclease and protease activity. They are both suitable for use in techniques requiring digestion of DNA in the recovery of intact RNA or where the integrity of structural proteins or enzymes must be maintained.

Applications have included:

• Nick translation
• DNA mapping
• Isolation of nuclear RNA and protein
• Plasmid construction
• RNA polymerase synthesis of RNA

Recombinant DNase I, Animal Origin Free (AOF) Bovine pancreas is a rich source of RNase which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase.

The processes involved in the production and isolation of recombinant DNase I (DR1/DR1S) are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.

Recombinant DNase I is suitable for such applications as:

• Removing genomic DNA from RNA preparations prior to RT-PCR
• Degradation of DNA templates after transcription reactions
• Removing unwanted DNA from samples prior to Northern blotting
• Removing DNA during biopharma and bioprocessing procedures