Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pac...read more
Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product. It can also be used in cell based TruPoint™ Caspase assays.
DELFIA cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.
Write a review
Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on March 30th 2014).
Join the Global SelectScience Community Today! It’s FREE!
Helping you make informed decisions about the latest lab products and technologies:
Be the first to learn about new technologies
10,000+ trusted product reviews from your peers
Compare 500,000 products and manufacturers
Get exclusive buying tips from experts
Find solutions fast: 10,000+ Application Notes, Webinars and Videos
Connect with 250,000 scientists... All in one place!