The DELFIA cAMP assay can be run with three different protocols - basic, simple, and high sensitivity. The basic and high sensitivity protocols involve adherent cells. In the simple protocol the determination is from cells in suspension, and all the steps from sample preparation to measurement of fluorescence are preformed on a DELFIA plate.
The sensitivity of the 384 kit is at least 0.1 ng/well (0.28 pmol/well) when using the simple assay protocol. When using the high sensitivity protocol the sensitivity is at least 0.1 pg/well (0.28 fmol/well).