BioSepra DEAE, CM, SP Trisacryl M, LS sorbents are high capacity ion exchange sorbents dedicated to a broad range of applications at large scale. Trisacryl ion exchangers are spherical semi-rigid microbeads of acrylic copolymers. The strongly bound ion exchange groups which are an integral part of the polymer structure are found within its three dimensional structure. Each Trisacryl polymer sub-unit possesses hydrophilic groups which ensure biocompatibility and prevent non-specific adsorption. Trisacryl ion exchangers are macroporous. Their open structure provides several advantages such as a rapid diffusion of macromolecules due to the high mass transfer, the easy access to ionizable groups and the absence of molecular sieving.
Capacity
The protein adsorption capacity of Trisacryl® sorbents is affected by general rules for the ion exchange mechanism. The capacity depends on a number of factors, including buffer pH and ionic strength, the nature of the counter-ion, and the characteristics of the protein such as molecular weight and isoelectric point. Variations in pH modify the ionization of the support groups and thus modify the available number of groups for protein adsorption. The adsorption capacity is particularly affected by the ionic strength and the pH of the buffer, which in turn modifies the net charge of the protein. Usually, an increase of ionic strength will result in a capacity decrease for a given protein. The adsorption capacity and flow rate are indicative features in the performance of an ion exchange support since they determine the productivity level of the support. These two factors are both taken in consideration when discussing productivity, since a flow rate increase often results in a capacity decrease. The adsorption capacity remains high as the flow rate increases. This phenomenon is attributed to the macroporous nature of the support, which provides a greater availability of the protein to ionizable groups.
Mechanical Stability
Trisacryl sorbents are semi-rigid and highly resistant to support collapse due to the three-dimensional structure obtained under specific polymerization conditions. Their physical and chemical features provide high flow rates in large columns.
Under high flow rates, the column equilibration, separation and regeneration may be performed rapidly and efficiently. The contact time of labile biochemical with the sorbent can be minimized, thus avoiding possible problem of denaturation.
Chemical Stability
Trisacryl sorbents are insoluble in all commonly used solvents in low and medium pressure chromatography, and have a good resistance to alkaline solutions. Additionally, the matrix is resistant to denaturing agents commonly used in biochemistry (8 M urea, 6 M guanidine hypochloride and other chaotropic agents) and to non-ionic detergents such as triton X-100, octylglucoside, and Tween 80. Trisacryl ion exchangers are acid-resistant, they can be used with inorganic acids (i.e. 1 M HCl), as well as organic acids, e.g. for the separation of peptides.
These sorbents are resistant to highly acidic solutions – contrary to polysaccharide-based supports – to highly alkaline solutions – contrary to most synthetic supports – and to enzymatic attack due to their synthetic origin.
All these properties are particularly important in large scale applications since they permit efficient sanitization and depyrogenation of the sorbent.
Thermal Stability
Trisacryl sorbents tolerate high thermal variations from -20 °C to +121 °C (the sorbents may be autoclaved during 20 min) without modification of their properties
Manufacturer Pall Life Sciences Products - Biopharmaceutical Division
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