CyGEL

Manufacturer Biostatus Limited  |   Model: CY10500  |  Available Worldwide
4.7
/
5.0
  |  5 reviews
A novel thermoreversible gel mountant for temporary immobilization and imaging.


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Average Rating: 4.7
5 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


Excellent product, affordable, easy to use, and gave great results.
Rating: 5.0

  • Application Area: Imaging non-adherent leukemia cell line for biosensor application.

"We used CyGel for imaging a K562 cell line to verify that our fluorophore-tagged biosensor is internalized by the cells. The product was very easy to use and worked according to the manufacturer’s description. There was no problem with CyGel interfering with our fluorescent signal using a standard fluorescent microscope for cell imaging. We think it was a very reasonable price for the ease of use and results we obtained. While we did not require follow up service, it was there for our assistance as needed."

Review date: 19 Sep 2016 | CyGEL
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Pittsburgh



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
I achieved reproducible results in imaging and rescuing zebrafish larva using CyGel.
Rating: 5.0

  • Application Area:Live Imaging of Zebrafish Larva

"Imaging mitochondrial dynamics requires that my sample be completely stationary and alive! CyGel allowed me to stabilize zebrafish larva and obtain time-lapse images of mitochondria. I was able to rescue the fish afterwards, so that fish could be imaged on multiple days if necessary."

Review date: 08 Aug 2016 | CyGEL
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The Francis Crick Institute



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Useful product
Rating: 3.7

  • Application Area:frog embryo research

"This is an effective product that we find is relatively easy to use. "

Review date: 13 Jun 2016 | CyGEL
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: BioImagingMW



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Reliable, user-friendly product
Rating: 5.0

  • Application Area:Live cell imaging by confocal microscopy

"We have used CyGEL to immobilize Drosophila pupae for live imaging using a confocal microscope. Embedding of samples in glass bottom dishes is carried out using a fluorescence stereomicroscope. We find CyGEL more convenient than LMP agarose because it saves the hassle to melt agarose and have a heating block or water bath nearby to keep agarose liquefied. The only utensil required is a small container with ice to keep CyGEL in its liquid form. Toxicity was not an issue since samples survived 3D time-lapse imaging for five days (see http://rsos.royalsocietypublishing.org/content/3/2/150517). Overall, we are very satisfied with this product."

Review date: 26 Feb 2016 | CyGEL
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Institute of Microbiology of the Academy of Sciences of the Czech Republic



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Confocal microscopy

"This is wonderful product to immobilize cells. It can be used also for immobilization of cells after cell sorting. Just sort cells on cool microscope slides and your cells will be immobilized during transport to confocal microscopy (as the slides will heat to room temperature). Just a very easy-to-use tool."

Review date: 09 Oct 2014 | CyGEL
A thermoreversible gel compatible with LIVE cells.

It can be used to immobilise non-adherent cells and beads by simple warming, and conversely allowing their recovery by simple cooling.

CyGEL™ is unusual in that it is a liquid when cold and a gel when warmed!

What are the key benefits?
CyGEL™ has many applications in imaging, flow cytometry and cell-based screening. It is compatible with LIVE cells, usefully allowing their immobilisation for analysis and subsequent release for ongoing experimentation.

Key benefits include:-
  • optically clear with low autofluorescence
  • controllable and rapid transition from liquid to gel
  • convenient immobilisation of non-adherent cells and beads
  • compatible with GFP and "in gel" fluorescent probes including DRAQ5™

Advantages:

  • allows rapid preparation of cells for gel mounting
  • permits long term analysis of non-adherent cells
  • acts as a support matrix
  • permits sterile, low temperature recovery of cells from gel
  • multiple assay formats and applications including apoptosis detection

Product Overview

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CyGEL

Manufacturer Biostatus Limited  |  Available Worldwide

4.7 / 5.0 | 5 reviews