Cyclic AMP EIA Kit by Cayman Chemical Co.

3.7

5.0

cAMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of certain ligands or hormones to their specific G-protein couple receptors activates GTP binding proteins (Gs or Gi) which either stimulate or inhibit adenylate cyclase. cAMP activates or in... Read more

The supplier does not provide quotations for this product through SelectScience. Browse these related products instead

Cyclic AMP EIA Kit

Reviews
Description

Average Rating: 3.7

1 Scientist has reviewed this product

3 out of 5
Ease of use

5 out of 5
After sales service

3 out of 5
Value for money

Write your own review

Thomas Pleli
Status:
Reviewer
Member since: 2009
Organization: University of Klinik Frankfurt

Ease of use

3 out of 5

After sales service

5 out of 5

Value for money

3 out of 5

Rating: 3.7

Review date: 10 Nov 2009 | Cyclic AMP EIA Kit

Binding of certain ligands or hormones to their specific G-protein couple receptors activates GTP binding proteins (Gs or Gi) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases. The Cayman Chemical cAMP assay is a competitive EIA that exhibits a limit of quantification of 0.1 pmol/ml (at 80% B/B0) for acetylated cAMP. Non-acetylated cAMP can be assayed with an IC50 of approximately 20 pmol/ml and a detection limit of 3 pmol/ml.

Product Overview