Overview Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. Now that recombinant proteins can be targeted to specific organelles, cells and tissues, recombinant apoaequorin may be even more useful for fine-tuning investigations of intracellular calcium mobilization. Furthermore the aequorin substrate, coelenterazine is freely permeant to cell membranes, facilitating the reconstitution of the aequorin complex in vivo.
Coelenterazine h is a derivative of native coelenterazine that is more sensitive to calcium, making it the ideal choice for luminescent calcium HTS.
Features and Benefits » Luminescent signal gives low compound interference » Fast, simple protocol » 20-fold more sensitive than native coelenterazine
Assay Principle The Coelenterazine/aequorin assay for calcium mobilization is very simple. A cell line that expresses an aequorin protein is pre-loaded with coelenterazine for 4-18 hrs. Then test compound is added and signal is read on an appropriate instrument.
Kit Contents » Coelenterazine h substrate
Instrument Requirements » Luminometer, Multimode Microplate Reader or CCD Camera » Luminometer with on board injectors
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