Cloning Vectors

Cloning Vectors

Agilent Technologies

Lambda Cloning Vectors pBC Phagemid Vectors pBlueScript II Phagemid System pCMV-Script System SuperCos I Vector ZAP Express System Lambda Cloning Vectors The Lambda ZAP-CMV vector allows produ...read more

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Description

 

Lambda Cloning Vectors
pBC Phagemid Vectors
pBlueScript II Phagemid System
pCMV-Script System
SuperCos I Vector
ZAP Express System
Lambda Cloning Vectors


The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved bluewhite color selection.

The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. SuperCos 1 is a novel, 7.9-kb cosmid vector that contains bacteriophage promoter sequences flanking a unique cloning site. This structure allows rapid synthesis of "walking" probes specific for the extreme ends of insert DNA. The SuperCos 1 vector is also engineered to contain genes for the amplification and expression of cosmid clones in eukaryotic cells. The ZAP Express vector combines the high efficiency of a lambda vector system with the versatility of a plasmid system. Fragments cloned into the ZAP Express vector can be excised with either R408, ExAssist helper phage or the Rapid Excision Kit to generate subclones in the pBK-CMV phagemid. pBK-CMV carries the neomycin-resistance gene for bacterial selection and for G418 resistance in eukaryotic cells.


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