IRDye® Click Chemistry reagents offer convenient, versatile and reliable methods for biomolecule labeling. With a variety of in vitro and in vivo applications, from biomarker isolation to assay devel...read more
IRDye® Click Chemistry reagents offer convenient, versatile and reliable methods for biomolecule labeling. With a variety of in vitro and in vivo applications, from biomarker isolation to assay development. Copper-free reagents are available for labeling live cells, and whole organisms. Click Chemistry has recently emerged as a convenient, versatile, and reliable method for labeling a wide variety of molecules for applications ranging from biomarker isolation to assay development. Click Chemistry utilizes pairs of reagents that exclusively react with each other and are effectively inert to naturally occurring functional groups such as amines.
Click Chemistry reactions can be categorized into two separate groups, copper-catalyzed or copper-free. Copper-catalyzed Click Chemistry is used for initiating reactions between azides and alkynes. Although they initiate and accelerate Click Reactions, copper catalysts are cytotoxic and inappropriate for use in living systems. To address this limitation, copper-free methods have been developed to allow Click Chemistry with live cells and whole organisms.
IRDye® Click Chemistry Reagents Features:
Versatile alternative to affinity-based detection
Chemoselective, easy-to-perform, high yielding reactions
Offer flexibility with a choice for copper-catalyzed and copper-free reactions
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