Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit by Chromatrap

It is essentially 96 separate Chromatrap® spin-columns in one device designed to enable researchers to perform many ChIP experiments in parallel. Assays are performed either by using hand-held multiple pipetting techniques or by using automated liquid handling robots for even higher throughputs.

The volume and dimensions of the wells in the microplate are very similar to that of the established Chromatrap® Spin-columns so the optimised spin-column protocol forms the basis of the approach to performing high throughput ChIP analysis in microplates.

Each well has its own porous disc at the bottom in which protein A or protein G has been covalently attached. The functionalised discs have the same chemistry and stoichiometry as the spin columns so all of the reagent volumes and timings can be directly inferred from the spin-tube protocol. The microplate is supplied with a close fitting collection plate to recover the retained chromatins at the elution step.

Protein A and Protein G variants of the Chromatrap® high-throughput microplate are currently being assessed at several beta sites using the existing spin-column protocol with the aim of developing an optimised high throughput protocol for broader use.