Chromatrap® Premium Pro-G ChIP Spin Column Sonication Kit by Chromatrap

The Chromatin immunoprecipitation (ChIP) assay is a technique used to investigate the interaction between proteins and DNA in the cell. For example, it may be used to determine whether proteins such as transcription factors and modified histones bind to a particular region on the endogenous chromatin of living cells or tissues.

In a ChIP assay, fragments of the DNA-protein complex (i.e. chromatin) are prepared in such a way so as to retain the specific DNA-protein interactions. These fragments are captured on an in inert porous solid phase disc using an immobilized antibody specific for the protein present in the complex. The isolated chromatin fraction can then be cleaned up, released into fresh buffers and treated to separate the DNA and protein components. The identity of DNA fragments isolated in connection with a particular protein can then be determined by Polymerase Chain Reaction (PCR), Real Time PCR, hybridization on microarrays, direct sequencing or other technologies used for identification of DNA fragments.

The kit contains sufficient Protein-G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.

This Premium kit also includes the following reference standards:
Positive Antibody Control: RNA Polymerase II (H - 244) Rabbit polyclonal
Negative Antibody Control: IgG from Rabbit serum 0.2µg/µl
Forward primer: GAPDH (human promoter region)
Reverse primer: GAPDH (human promoter region)


Chromatrap® Premium Pro-G ChIP Spin Column Sonication Kit Contents:

• Chromatrap Pro-G spin columns
• Column conditioning buffer
• Wash Buffer 1
• Wash Buffer 2
• Wash Buffer 3
• 1.3M Glycine
• Lysis Buffer
• Elution solution
• 0.1M NaOH
• 5M NaCl
• 1M NaHCO3
• Hypotonic buffer
• Protease Inhibitor Cocktail (PIC)
• Proteinase K stop solution
• Proteinase K
• 1.5ml Collection tube