Chromatrap® Enzymatic Shearing Kit by Chromatrap

Chromatrap® enzymatic shearing kit supplies all the necessary reagents and buffers for up to 10 chromatin preparations.

The success of a ChIP assay is highly dependent on the quality of chromatin prepared. Chromatrap® enzymatic shearing kit allows you to determine optimal shearing conditions for your chromatin preparations and can supply you with enough chromatin to perform up to 24 ChIPs if using standard Chromatrap® ChIP spin column kit or up to 96 IP’s if using Chromatrap® ChIP 96 High throughput microplate.


Chromatrap® Enzymatic Shearing Kit Contents:

• 1.3M Glycine 20mls
• Hypotonic Buffer 10mls
• Digestion Buffer 10mls
• Enzymatic Stop Solution 200µl
• Lysis Buffer 10mls
• 5M NaCl 500µl
• 1M NaHCO3 500µl
• Shearing Cocktail 100µl
• Protease Inhibitor Cocktail (PIC) 100µl
• Proteinase K 50µl
• Proteinase K Stop Solution 100µl