CHO-K1 (+Gqi5) AequoScreen® Parental cell line


PerkinElmer, Inc.

AequoScreen® Parental cells stably expressing the mitochondrially targeted apoaequorin and the G-protein Gqi5 for flash-luminescence assay. Recombinant, in CHO-K1 host cell. GPCRs of your choice ca...read more

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Description

 

AequoScreen® Parental cells stably expressing the mitochondrially targeted apoaequorin and the G-protein Gqi5 for flash-luminescence assay.
Recombinant, in CHO-K1 host cell. GPCRs of your choice can be transiently / stably transfected into these cell lines. Following GPCR stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal. Two vials of cryopreserved cells are shipped per order.

Feature/Benefits:

  • Functional, homogenous and generic assay platform for Gi coupled GPCRs
  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z, CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput

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