AequoScreen® Parental cells stably expressing the mitochondrially targeted apoaequorin and the G-protein Gqi5 for flash-luminescence assay. Recombinant, in CHO-K1 host cell. GPCRs of your choice can be transiently / stably transfected into these cell lines. Following GPCR stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal. Two vials of cryopreserved cells are shipped per order.
Feature/Benefits:
Functional, homogenous and generic assay platform for Gi coupled GPCRs
Fully automatable and miniaturizable to 1536
Enables reduction in reagents and consumable costs
High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
Robust assay (Z, CV values, solvent resistance)
Reduces the number of false positives from autofluorescent compounds in screening libraries
Option to work with cells in suspension to increase throughput
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