Chirascan™ Q100 Circular Dichroism Spectrometer by Applied Photophysics Ltd

The Chirascan Q100 Circular Dichroism (CD) Spectrometer offers comprehensive insight into the Higher Order Structure (HOS) characteristics of complex biomolecules. High-performance CD spectrometry and reproducible robotics combine to produce quality data compatible with the most strict statistical analysis techniques. The outcome: objective, statistically-validated HOS comparisons. Fully integrated for unmanned operation, the Chirascan Q100 saves days of operator time, analyzing 48 buffer-sample pairs in 24 hours whereas a skilled operator can process only up to 14 samples per day on a manual system. 

Ready to run

The Chirascan systems are provided with the features and accessories needed for acquisition of superior quality data from ‘day one’.

Molecular Sieve, Activated Charcoal Filter

• Removes common gas impurities

Active Nitrogen Management System

• Software-controlled
• Regulates purge gas consumption

Air-Cooled Xenon Lamp

• Up-time recorded
• Software-controlled

Monochromator

• Dual polarizing prisms maximize light throughput
• Generates horizontally, linearly polarized monochromatic light

Photoelastic Modulator

• Converts horizontally polarized light to circularly polarized light. Alternates between left- and right-handed circular polarized light

Integrated Autosampler

• Precise liquid handling and reproducibility
• Eliminate sample handling errors
• Temperature-controlled storage maintains sample integrity

Temperature-Controlled Sample Chamber

• Continuous temperature ramps (single sample mode)
• Consistent analytical conditions

Water Circulator

• Dissipates heat from sample chamber and sample storage Peltiers

Avalanche Photodiode Detector

• Maximum sensitivity (high signal: noise)

Optics-Based, Multiwave­Length Calibration

• For CD accuracy at every wavelength

Fixed Flow Cells

• Eliminates errors of cuvette handling
• Choice of pathlength to optimize concentration and absorbance
• Recognized by Chirascan Control to choose run/wash/dry protocols

Cuvettes and Holders

• Selected for far- and near-UV CD analysis of biomolecules (single sample mode)

Control and Analysis Software

• Saves time with scheduled start-up/shutdown of lamp and N₂ supply
• Easily defined run parameters and store standard protocols
• Ensures O₂-free conditions with N₂ purge
• Fail-safe lamp switch-off if N₂ flow drops
• Recognizes flow cell to choose ideal run/wash/dry protocol

HOS Comparison Software

• Create statistically-validated ­comparisons

Global Thermodynamic Analysis

• Obtain melting points and enthalpies from multi-wavelength, thermal denaturation experiments (single sample mode)