Chirascan™ Circular Dichroism Spectrometer

Chirascan™ Circular Dichroism Spectrometer

Applied Photophysics Ltd

Chirascan is a powerful, modern circular dichroism (CD) spectrometer and thus a fast, sensitive, direct probe of the secondary structure, or conformation, of bio-molecules. Provides det...read more

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Description

 

Chirascan is a powerful, modern circular dichroism (CD) spectrometer and thus a fast, sensitive, direct probe of the secondary structure, or conformation, of bio-molecules.

  • Provides detailed structure analysis of biomolecules like proteins
  • Outstanding sensitivity delivers accurate and precise results quickly
  • Exceptional signal-to-noise ratio
  • Powerful software for easy set-up, display and analysis
  • Comprehensive set of accessories maximizes scope of applications
  • Low usage of consumables minimizes cost of ownership

One of the principal applications of Chirascan is in determining the secondary structure of proteins and it is also used to investigate their tertiary structure by measuring the CD induced in the aromatic side-chains and disulphide bonds of the peptide backbone.

Protein secondary and tertiary structures change under stress, for example with temperature or pH, and circular dichroism is used to assess conformational stability with changing environment. Used in this way, it has an important role to play in biotherapeutic drug development and formulation testing, where it can help to optimise buffer conditions that enhance product longevity. Circular dichroism is also used in bio-comparability studies to demonstrate conformational equivalence of material from different processes or sources and for determining that novel proteins are correctly folded. Today, the performance of Chirascan is the standard by which CD spectrometers are judged.

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