CHIP-1000

CHIP-1000

Shimadzu Scientific Instruments Inc.

The CHIP-1000 precisely and accurately delivers pico-liter volumes of enzymes, antibodies or MALDI-TOF matrices by employing state-of-the-art chemical inkjet printing technology. It is ideal for such ...read more

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Description

 

The CHIP-1000 precisely and accurately delivers pico-liter volumes of enzymes, antibodies or MALDI-TOF matrices by employing state-of-the-art chemical inkjet printing technology. It is ideal for such applications as MALDI tissue imaging/profiling, Nanowestern analyses and multiple reagent printing onto various target substrates.

Features of the CHIP-1000
Exceptional Reproducibility
Piezoelectric technology enables highly reliable micro-dispensing and exceptional drop-on-drop reproducibility. The diameter of each capillary is only 55um, and volumes as low as 100pl can be dispensed. 4 piezoelectric heads can deliver 4 different solutions to specific locations on a target surface. This also ensures that incompatible reagents for different applications will not come into contact.

Camera/Built-in Scanner
The camera is employed to optimize and monitor droplet formation, while the visible light scanner is used to identify target areas on the tissue surface for reagent delivery, aid visualization of the deposited arrays and create a target map for printing.

Sample Stage
Accurate stage positioning supports a high level of reproducibility of dispensing position precision. Two MALDI target plates (four glass slides) can be mounted.

Image Import Functionality – Enables precise delivery of reagents to areas of interest.

Software
Data analysis can be performed in proprietary Shimadzu MALDI-MS Imaging software. Information about the coordinates of the dispensing position is output in CSV file format by the export function. Loading this information to a MALDI-MS instrument enables MALDI-MS analysis with respect to each dispensing position.

The export feature of all AXIMA systems permits the easy transfer of data from the LaunchPad™ instrument software to a wide variety of alternative processing packages, including BioMap.

Applications
Established applications for the CHIP include on-membrane protein digests, followed by direct on-membrane MALDI PMF and MSMS protein identification. CHIP nano-Western blotting offers superb value and a highly sensitive alternative approach to standard Western blotting, enabling multiple experiments per membrane.

In addition, protein solutions, such as trypsin and a variety of MALDI matrices, can be printed with the CHIP. Large matrix arrays can be generated to cover the entire surfaces of tissue sections (imaging) or smaller arrays can be used to study the differences between a many different areas within the tissue (profiling).

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