CFX384 Touch™ Real-Time PCR Detection System by Bio-Rad

Use the CFX384 Touch Real-Time PCR Detection System for precise, reproducible real-time PCR. The system is composed of the CFX384 Optical Reaction Module and the C1000 Touch Thermal Cycler Chassis. The module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Chassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.

Features and Benefits

• Detection of up to 4 targets per well, plus a channel dedicated to singleplex FRET
• Protocol autowriter that generates an optimal protocol for your reaction components
• Thermal gradient feature that identifies optimal annealing temperature in a single run

The CFX384 Touch Real-Time PCR Detection System has six independently controlled thermal electric units providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity).

The optical system, with five filtered LEDs and five filtered photodiodes, collects data from all wells, detecting up to four targets per well. For single-color FAM and SYBR^® Green I, the fast scan option reads single-channel fluorescence in all 384 wells in just 8 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.

The large touch-screen interface has intuitive graphical programming and displays data traces in real time. The programmable thermal gradient feature can test eight temperatures at once to quickly determine the optimal annealing temperature, and the protocol autowriter quickly optimizes reactions, generating an optimal protocol based on your polymerase, primers, and product length.

Product Components

• CFX384 Optical Reaction Module
• C1000 Touch Thermal Cycler Chassis
• USB cable
• Instruction manual

Configuration Options

The following modules can be used with the C1000 Touch Thermal Cycler Chassis:

*Starter package contains SsoAdvanced™ Universal SYBR^® Green Supermix ( 1725270 ), Microseal^® 'B' Adhesive Seals ( MSB1001 ), and Hard-Shell^® Low-Profile Thin-Wall 96-Well Skirted PCR Plates ( HSP9601 ) or Hard-Shell Thin-Wall 384-Well Skirted PCR Plates ( HSP3805 ).

The CFX384 Touch Real-Time PCR Detection System can be configured with other PCR instruments, allowing stand-alone or software control. Instrument permutations allow up to four different CFX Connect™, CFX96, or CFX384 Systems to be controlled by one PC.

Combine any two CFX Real-Time PCR Detection Systems with the CFX Automation System II , our robotic plate handler, to create a high-throughput, automated real-time PCR system with a compact footprint.

Service Plans and Validation Tools for CFX Systems

• Bio-Rad Expert Care Service offers several service plans to keep your CFX Systems running, including ensuring that your real-time PCR systems are functioning optimally
• Certify system installation and validate system performance with CFX System IQ/OQ Service
• Verify the performance of your CFX Systems with Thermal Validation Services and the CFX Qualification Plates

Related Products

• C1000 Touch Thermal Cycler Chassis ( 1841100 )
• PX1™ PCR Plate Sealer
• PCR plastics optimized for CFX Real-Time PCR Detection Systems:
  • Hard-Shell 384-Well Skirted PCR Plates
  • Microseal 'B' Adhesive Seals ( MSB1001 )
  • Optically Clear Heat Seals ( 1814030 )
• CFX Maestro Software, Security Edition , for compliance with U.S. FDA 21 CFR Part 11 regulations
• CFX Qualification Plate for performance verification, 384-well  ( 1845099 )

Related Categories

• Real-Time PCR Detection Systems
• PCR Reagents and qPCR Reagents
• PCR Plastic Consumables
• PCR Seals
• PCR Analysis Software

Online Resources

• Real-Time PCR Detection Systems Selection Chart
• qPCR/Real-Time PCR
• qPCR Instrumentation