Status:
ReviewerMember since: 2019
Organization: MIT
Easy to use, consistent results.
Application Area: Intestinal organoids
"Extremely easy to handle, consistent, and reproducible results. Can prepare standard control when purchasing standard ATP."
Status:
ReviewerMember since: 2019
Organization: BioIVT
Most used reagent in our lab currently.
Application Area:Measure viability in 3D tumour assay
"Easesy to use - defrost and add directly to the cells, leave for only 15mins and then read. Simple and reliable."
Status:
Advanced ReviewerMember since: 2015
Organization: The Institute of Cancer Research
Highly recommended.
Application Area:Cell viability
"Easy to use, very robust, but expensive. Will recommend to others."
Status:
ReviewerMember since: 2017
Organization: FAU
Excellent product, use it almost on a daily basis.
Application Area:Spheroid and tumoroid toxicity.
"CellTiter Glo 3D is very easy to use and works very efficiently, yielding high quality and reproducible results. When we moved to 3D cell culture technologies, there weren't many products available for efficient use in these systems, so we were very happy to be able to use the product early on."
Status:
Advanced ReviewerMember since: 2015
Organization: German Cancer Research Center (DKFZ)
Best cell assay for 3D cell culture!
Application Area:Cell viability assessment of spheroids
"CellTiterGlo3D is the only assay that gives reproducible results on spheroid cultures. The results are always very precise, so that one can detect even minor changes in cell viability, which is not detectable with other products."
The CellTiter-Glo® 3D Cell Viability Assay is a homogeneous method to determine the number of viable cells in 3D cell culture based on quantitation of the ATP present, which is a marker for the presence of metabolically active cells.
This ready-to-use reagent is based on the original CellTiter-Glo® Luminescent Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent. The CellTiter-Glo® 3D Cell Viability Assay is formulated with more robust lytic capacity and is designed for use with microtissues produced in 3D cell culture.
The homogeneous assay procedure involves addition of a single reagent (CellTiter-Glo® 3D Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. This assay is compatible with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS). The CellTiter-Glo® 3D Assay has been used successfully with 3D microtissue cell culture produced via hanging-drop plates, ultra-low attachment plates, Matrigel®-coated plates, agarose-coated plates, cultures suspended in methylcellulose and Alvetex® plates.
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