Cells-to-cDNA™ II Kit by Thermo Fisher Scientific Invitrogen
Manufacturer Thermo Fisher Scientific Invitrogen
The Ambion® Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr. No RNA isolation is required.
This kit contains sufficient reagents for 40 reactions and is ideal for those who want to perform reverse transcription reactions on small numbers of cells, numerous cell samples, or for scientists who are unfamiliar with RNA isolation.The Cells-to-cDNA™ II Kit (patent pending) integrates RNase inactivation and DNase I treatment into an RT-PCR compatibl... Read more
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Cells-to-cDNA™ II Kit
Average Rating: 2.0
1 Scientist has reviewed this product
Status:
Advanced Reviewer
Member since: 2007
Organization: King Abdullah International Medical Research Center
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Rating: 2.0
Application Area: RT PCR
"Cells-to-cDNA kit is designed to minimise steps in carrying out conventional end point PCR. It has two options, one step and two steps RT-PCR. In One step, you can only carry out endpoint PCR whereas in two steps, the cDNA can be used for other applications. However, the overall process of optimization with Armored RNA control in pilot experiments did not work very well with me. Also, it remains to be seen if this kit can work with homogenized tissue from different sources. The use of conventional RNA extraction and cDNA preparation kits does not feel bad after using this kit which promises ease but actually does not. I worked with HCT-8 cells and used random primers for preparing cDNA. RT-PCR was done using CTNNB and GAPDH primers. It did not work. The Armored RNA control did work in the pilot study but the controls were not satisfactory."
Review date: 18 Feb 2014 | Cells-to-cDNA™ II Kit
The Ambion® Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr. No RNA isolation is required.
This kit contains sufficient reagents for 40 reactions and is ideal for those who want to perform reverse transcription reactions on small numbers of cells, numerous cell samples, or for scientists who are unfamiliar with RNA isolation.
The Cells-to-cDNA™ II Kit (patent pending) integrates RNase inactivation and DNase I treatment into an RT-PCR compatible cell lysis buffer, eliminating RNA isolation altogether. Most cell lysis buffers contain strong denaturants that if carried over into enzymatic reactions would inhibit or inactivate most enzymes. If a lysis buffer without strong denaturants is used, endogenous RNase can quickly degrade cellular RNA.
The Cells-to-cDNA™ II Kit contains a novel Cell Lysis Buffer that inactivates endogenous RNases without compromising downstream enzymatic reactions. After inactivation of RNases, the cell lysate can be directly added to a cDNA synthesis reaction. Cells-to-cDNA™ II is compatible with both one-step and two-step RT-PCR protocols.
Cells-to-cDNA™ II Kit Features:
This kit contains sufficient reagents for 40 reactions and is ideal for those who want to perform reverse transcription reactions on small numbers of cells, numerous cell samples, or for scientists who are unfamiliar with RNA isolation.
The Cells-to-cDNA™ II Kit (patent pending) integrates RNase inactivation and DNase I treatment into an RT-PCR compatible cell lysis buffer, eliminating RNA isolation altogether. Most cell lysis buffers contain strong denaturants that if carried over into enzymatic reactions would inhibit or inactivate most enzymes. If a lysis buffer without strong denaturants is used, endogenous RNase can quickly degrade cellular RNA.
The Cells-to-cDNA™ II Kit contains a novel Cell Lysis Buffer that inactivates endogenous RNases without compromising downstream enzymatic reactions. After inactivation of RNases, the cell lysate can be directly added to a cDNA synthesis reaction. Cells-to-cDNA™ II is compatible with both one-step and two-step RT-PCR protocols.
Cells-to-cDNA™ II Kit Features:
- No RNA purification required
- From cells in culture to cDNA in less than 2 hr
- Detect rare messages in as few as 1 cell
- Ideal for labs not equipped for RNA isolation
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