Cell Proliferation Dye eFluor® 670 is a red fluorescent dye that can be used to monitor individual cell divisions. This fluorescent dye binds to any cellular protein containing primary amines, and as cells divide, the dye is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye. Up to 6 generations may be visualized. Cell Proliferation Dye eFluor® 670 can also be used for long term tracking of labeled cells. Analysis using two-parameter plots may provide better resolution of each generation, especially between undivided cells and the first generation. Cells labeled with Cell Proliferation Dye eFluor® 670 may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixati...Read more
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