The Guava® Caspase 3/7 and 8 Assays employ a FLICA (Fluorescent Labeled Inhibitor of Caspases) reagent that specifically identifies active caspase 3 and 7 molecules or caspase 8 molecules.
Non-cytotoxic, cell permeant dyes elimiate lysis and permeabilization procedures. Each FLICA is comprised of three subunits:
A caspase-specific amino acid inhibitor containing a recognition sequence for caspase 3/7 (DEVD) or caspase 8 (LETD); a fluoromethylketone moiety (FMK) that forms a covalent linkage with the active enzyme; and a carboxyfluorescein (FAM) reporter.
The nuclear DNA stain is also included in the assay to simultaneously evaluate membrane integrity and cell viability. For use with all instruments.
Receive your quote directly from EMD Millipore, a division of Merck KGaA, Darmstadt, Germany for Caspase 3/7 Reagent Kit (100 tests)
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Member since: 2011
Organization: UNIVERSITY OF MARYLAND