Caspase 3/7 Reagent Kit (100 tests) by MilliporeSigma
Manufacturer MilliporeSigma
Caspase 3/7 Reagent Kit (100 tests)
Average Rating: 3.7
1 Scientist has reviewed this product
Status:
Member since: 2011
Organization: UNIVERSITY OF MARYLAND
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Rating: 3.7
"Works well but expensive."
Review date: 01 Jul 2011 | Caspase 3/7 Reagent Kit (100 tests)
The Guava® Caspase 3/7 and 8 Assays employ a FLICA (Fluorescent Labeled Inhibitor of Caspases) reagent that specifically identifies active caspase 3 and 7 molecules or caspase 8 molecules.
Non-cytotoxic, cell permeant dyes elimiate lysis and permeabilization procedures. Each FLICA is comprised of three subunits:
A caspase-specific amino acid inhibitor containing a recognition sequence for caspase 3/7 (DEVD) or caspase 8 (LETD); a fluoromethylketone moiety (FMK) that forms a covalent linkage with the active enzyme; and a carboxyfluorescein (FAM) reporter.
The nuclear DNA stain is also included in the assay to simultaneously evaluate membrane integrity and cell viability. For use with all instruments.
Non-cytotoxic, cell permeant dyes elimiate lysis and permeabilization procedures. Each FLICA is comprised of three subunits:
A caspase-specific amino acid inhibitor containing a recognition sequence for caspase 3/7 (DEVD) or caspase 8 (LETD); a fluoromethylketone moiety (FMK) that forms a covalent linkage with the active enzyme; and a carboxyfluorescein (FAM) reporter.
The nuclear DNA stain is also included in the assay to simultaneously evaluate membrane integrity and cell viability. For use with all instruments.
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