Thermo Scientific™ CarboPac® PA200 Carbohydrate Column by Thermo Fisher Scientific

Fast, pH-Stable Separation of Oligosaccharides

A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.

CarboPac® PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.


CarboPac® PA200 Carbohydrate Column Features:

• Sensitive, direct detection with no derivatization, using pulsed amperometric detection
• Very high-efficiency separations
• Separations based on size, charge, degree of branching, and linkage isomerism
• Low flow rate saves on eluent consumption
• Does not require derivatization

Column Details:

• Length: 50, 250 mm
• ID: 3.0 mm