Capturem™ Protein A Antibody Purification Kits by Takara Bio

Rapid Antibody Purification Kits—Capturem Protein A Miniprep

Capturem Protein A Miniprep columns provide rapid, high-quality, small-scale purification and screening of monoclonal and polyclonal antibodies. These miniprep columns combine the specific antibody-binding properties of Protein A with novel Capturem technology to provide high-capacity, membrane-based affinity purification. These columns are convenient, powerful, and efficient tools for small-scale antibody purification, and offer the following advantages:

• Fast and convenient: purify antibodies in 5 minutes at room temperature
• High quality: short residence times reduce the possibility of aggregation or loss of activity
• High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
• Flexible: purify a wide range of antibodies

Protein A is a cell wall protein from the bacterium Staphylococcus aureus with a high affinity for the FC region of monoclonal and polyclonal IgG-type antibodies from several different species, e.g., mouse, rat, and human antibodies. Capturem kits use a novel high-capacity membrane-based affinity technology that has previously been used to purify highly concentrated his-tagged proteins in just minutes at room temperature, via miniprep columns, maxiprep columns, or 96-well plates.

Features:

• No-waiting workflow: 5-minute, room-temperature protocol
• High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
• High concentration: extremely small elution volumes yield highly concentrated antibodies
• High quality: short residence times reduce the possibility of aggregation or loss of activity
• Versatile: purify a wide range of antibodies
• Single-use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover

Applications:

• Antibody purification
• Antibody screening
• Immunoprecipitation