The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately).
pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_
Novel purification tag combines small size, gentle elution and high-affinity binding
Easy tag removal resulting in native protein
System consists of vectors, competent cells and resin