Overview and Description
The DiscoveRx Calcium No Wash (Ca NW) Assay kit provides a fast, reliable, low cost alternative to traditional wash and quench-based assays for detection of changes in intracellular calcium in response to GPCR activation.
Features and Benefits
» Low cost alternative
» High sensitivity and fluorescence intensity
» No interference from quenchers binding to target proteins or ligands
» Ready-to-use probenecid solution for enhanced dye retention
Calcium Flux Assay Principle
This kit provides a homogenous assay format for detection of calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with a calcium-sensitive dye and then treated with compound. Upon stimulation, the receptor signals release of intracellular calcium, which then causes the dye to fluoresce. The signal generated in the assay is read using fluorescent plate readers that possess an injector for addition of agonist and is capable of a rapid read of the resulting change in fluorescence. The assay has been designed to accommodate both 96 well and 384 well analysis.
Kit Contents
» Calcium No Wash Dye
» Dye Loading Buffer
» Probenecid
Instrument Requirements
» Fluorescent Intensity Detection
» Fluorescence reader with on board injectors