C-DiGit® Blot Scanner for Chemiluminescent Western Blots by LI-COR Biosciences

"Cost effective: excellent. Reproducible results: excellent. Very easy to use: excellent. After sales care: Excellent, reachable, helpful."

"I run many mini western blots and use the C-DiGit to image the blots. It is very easy to use, very small, and always produces a quality image. The one thing that I would change would be increaseing the imaging surface area so that it can accommodate larger blot sizes (although there is probably another model for that). Overall I am very happy with the ease of use of this product and the results it produces!"

"Very easy to use and worth the money. Selection of correct chemiluminescent reagent is a key."

"I love this machine - small but effective. I made several hundred of western blots and working well, it is easy to use and looks nice on the office table as well :) Also, the customer care is very nice - the people from LiCor are coming time by time and they can also solve some western-problems, so I am very happy with it. The only disadvantage is the software, which do not run on the new versions of Windows - the drivers are no available, so, then it was little problem if the windows is updated."

"A lot of the reviews on this site seem like they're written by a marketing company. Anyway, here's a candid view from someone that has regularly used this for two years. For one, the machine is very easy to use. So why did I rate it 3 stars in that category? Well, people who routinely do very long exposures on film are going to wonder why it's not working out for them, and I don't think Licor has been forward enough about the sensitivity, which, in my view, is 40-60% less sensitive than film. We had a lot of people try the machine once and swear to never use it again. An 11 minute exposure on the machine, even when a blot is directly placed on the machine with no plastic wrap, is indisputably not equivalent to an 11 minute exposure on film. That being said, this problem is easily overcome by using stronger substrates (which can also be diluted with water to your likeness!) and/or by improving their western blot technique. It would be wise for Licor to be more forward about this and help users view their fussy blots on the machine from the get-go. They have resources, but they're too minimalistic. Once you know the limitations and tools available, there shouldn't be very many blots you can't get to work on this. I've successfully blotted HIF-1alpha from a whole cell lysate on this machine, which is notoriously difficult to detect. And at the end of the day, you'll get more accurate quantitative data than you would with film."

"The product satisfies all the exigence of our laboratory. It was more precise and efficient than classic bioluminescence in dark room."

"This machine is fantastic! It totally beats the old school method of developing film. It is simple to use and I can count on it to consistently image nice blots!"

"We are using the C-DiGit Blot Scanner routinely in our laboratories for the development and characterization of novel antibodies. Setting up the scanner is straightforward and it takes only little time to get started and to produce high-quality western blot images. The software interface is very easy to use. In particular, we like the possibility to choose different "exposure" times. This allows us to screen several antibodies on one single blot in a fraction of time compared to using several ECL films and traditional film development methods. The instant blot digitization on the scanner and the ability to perform quantification further eliminates the need for other equipment and software. For us, the C-DiGit is the perfect solution as it reduces our costs for reagents as well as waste disposal and saves significant space. We therefore highly recommend this scanner for western blot applications. "

"This is a great little scanner. We find this helps with faster throughput and is certainly cheaper and easier than film. The sensitivity is very good, so that we only occasionally need to go back to film for a particularly fussy antibody."