The Brilliant II Fast QPCR Master Mix is a single-tube reagent designed for performing quantitative PCR amplifications with an accelerated cycling protocol that completes 40 cycles of PCR in approximately 48 minutes, yielding results in half the time of standard QPCR protocols. The master mix formulation and fast cycling protocol were developed together to create a system for completing QPCR reactions in less time without compromising target detection sensitivity, specificity, or reproducibility. The Brilliant II Fast QPCR master mix has been successfully used with fluorescent TaqMan probes to amplify and detect a variety of DNA targets, including genomic DNA, plasmid DNA, and cDNA.
Use with fluorescent probes to amplify and detect a variety of DNA targets, including genomic DNA, plasmid DNA, and cDNA
Accelerated cycling protocol that completes 40 cycles of PCR in approximately 48 minutes
Rapid HotStart capability to minimize non-specific product formation while reducing run time
Improved performance with earlier Ct detection across a wide dynamic range
Increased sensitivity of detection to low copy numbers
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